Antifungal, anti-oomycete and phytotoxic effects of volatile organic compounds from the endophytic fungus Xylaria sp strain PB3f3 isolated from Haematoxylon brasiletto
Por:
Sanchez-Ortiz, B. L., Sanchez-Fernandez, R. E., Duarte, G., Lappe-Oliveras, P., Macias-Rubalcava, M. L.
Publicada:
1 may 2016
Resumen:
AimsTo determine the antifungal, anti-oomycete and phytotoxic activity;
and chemical composition of the volatile organic compounds (VOCs)
produced by endophytic fungus Xylaria sp. PB3f3 isolated from
Haematoxylon brasiletto Karst.
Methods and ResultsBioactivity and chemical composition of the VOCs from
Xylaria sp. PB3f3 were established by using simple and multiple
antagonism bioassays, and gas chromatography/mass spectrometry,
respectively. The results showed that Xylaria sp. PB3f3 inhibited the
growth of the oomycetes Pythium aphanidermatum (783%), Phytophthora
capsici (483%), and the fungi Alternaria solani (245%) and Fusarium
oxysporum (242%), in multiple antagonism bioassays. Volatile organic
compounds, produced at 20 and 30days of fungal growth, inhibited root
elongation on Amaranthus hypochondriacus (276%) and on Solanum
lycopersicum (532%). Forty VOCs were identified at 10, 20 and 30days in
Xylaria sp. PB3f3 cultures. The compounds with the highest fibre
affinity were: 3-methyl-1-butanol and thujopsene, at 10days of fungal
growth; an unidentified amine and 2-methyl-1-butanol at 20days; and
2-methyl-1-propanol at 30days. In the gas phase assay method
2-methyl-1-propanol and 2-methyl-1-butanol showed significant inhibitory
effects on root elongation and germination of Am.hypochondriacus and
S.lycopersicum.
ConclusionsXylaria sp. PB3f3 and its VOCs showed significant phytotoxic
effects on root elongation and germination of Am.hypochondriacus and
S.lycopersicum.
Significance and Impact of the StudyThe genus Xylaria produces a great
variety of secondary metabolites, but, up date, there are no reports of
the identification of bioactive volatile compounds. Thus, Xylaria sp.
PB3f3 and its VOCs are a possible candidate for the biological control
of weeds.
Filiaciones:
Sanchez-Ortiz, B. L.:
Univ Nacl Autonoma Mexico, Dept Nat Prod, Inst Quim, Ciudad Univ, Coyoacan, Mexico
Sanchez-Fernandez, R. E.:
Univ Nacl Autonoma Mexico, Dept Nat Prod, Inst Quim, Ciudad Univ, Coyoacan, Mexico
Duarte, G.:
Univ Nacl Autonoma Mexico, Fac Quim, Unidad Serv Apoyo Invest, Ciudad Univ, Coyoacan, Mexico
Lappe-Oliveras, P.:
Univ Nacl Autonoma Mexico, Inst Biol, Dept Bot, Ciudad Univ, Coyoacan, Mexico
Macias-Rubalcava, M. L.:
Univ Nacl Autonoma Mexico, Dept Nat Prod, Inst Quim, Ciudad Univ, Coyoacan, Mexico
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