The Sinorhizobium meliloti glyoxylate cycle enzyme isocitrate lyase (AceA) is required for the utilization of poly-beta-hydroxybutyrate during carbon starvation
Por:
Augusto Ramirez-Trujillo, Jose, Dunn, Michael F., Suarez-Rodriguez, Ramon, Hernandez-Lucas, Ismael
Publicada:
1 jun 2016
Categoría:
Applied Microbiology and Biotechnology
Resumen:
The glyoxylate cycle is an anaplerotic pathway of the tricarboxylic acid
cycle that allows bacteria to grow using acetate, fatty acids, or
poly-beta-hydroxybutyrate (PHB). In Sinorhizobium meliloti, activities
of the glyoxylate cycle enzymes isocitrate lyase (AceA) and malate
synthase (GlcB) are present during growth on these kinds of carbon
sources, but a study of the importance of these enzymes in utilizing
these carbon compounds under starvation conditions has not been done. We
therefore evaluated the role of AceA and GlcB in the utilization of PHB
by determining the PHB degradative and growth capacities of the S.
meliloti wild type and aceA and glcB mutants under carbon starvation
conditions in culture. We found that only the aceA gene product was
essential for bacterial growth and PHB degradation under these
conditions, presumably by generating succinate from the acetyl-CoA
derived from PHB catabolism, thus allowing the cells to grow in the
absence of an external carbon source.
Filiaciones:
Augusto Ramirez-Trujillo, Jose:
Univ Autonoma Estado Morelos, Lab Fisiol Mol Plantas, Ctr Invest Biotecnol, Av Univ 2001, Cuernavaca 62209, Morelos, Mexico
Dunn, Michael F.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62210, Morelos, Mexico
Suarez-Rodriguez, Ramon:
Univ Autonoma Estado Morelos, Lab Fisiol Mol Plantas, Ctr Invest Biotecnol, Av Univ 2001, Cuernavaca 62209, Morelos, Mexico
Hernandez-Lucas, Ismael:
Univ Nacl Autonoma Mexico, Dept Mol Microbiol, Inst Biotecnol, Av Univ 2001, Cuernavaca 62210, Morelos, Mexico
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