Resin glycosides from Ipomoea wolcottiana as modulators of the multidrug resistance phenotype in vitro
Por:
Corona-Castaneda, Berenice, Rosas-Ramirez, Daniel, Castaneda-Gomez, Jhon, Alejandro Aparicio-Cuevas, Manuel, Fragoso-Serrano, Mabel, Figueroa-Gonzalez, Gabriela, Pereda-Miranda, Rogelio
Publicada:
1 mar 2016
Resumen:
Recycling liquid chromatography was used for the isolation and
purification of resin glycosides from the CHCl3-soluble extracts
prepared using flowers of Ipomoea wolcottiana Rose var. wolcottiana.
Bioassay guided fractionation, using modulation of both antibiotic
activity against multidrug-resistant strains of Gram-negative bacteria
and vinblastine susceptibility in breast carcinoma cells, was used to
isolate the active glycolipids as modulators of the multidrug resistance
phenotype. An ester-type dimer, wolcottine I, one tetra- and three
pentasaccharides, wolcottinosides I-IV, in addition to the known
intrapilosin VII, were characterized by NMR spectroscopy and mass
spectrometry. In vitro assays established that none of these metabolites
displayed antibacterial activity (MIC > 512 mu g/mL) against
multidrug-resistant strains of Escherichia coli, and two nosocomial
pathogens: Salmonella enterica serovar Typhi and Shigella flexneri;
however, when tested (25 mu g/mL) in combination with tetracycline,
kanamycin or chloramphenicol, they exerted a potentiation effect of the
antibiotic susceptibility up to eightfold (64 mu g/mL from 512 mu g/mL).
It was also determined that these non-cytotoxic (CI50 > 8.68 mu M)
agents modulated vinblastine susceptibility at 25 mu g/mL in
MFC-7/Vin(+) cells with a reversal factor (RFMcF-7/Vin+) of 2-130 fold.
(C) 2016 Elsevier Ltd. All rights reserved.
Filiaciones:
Corona-Castaneda, Berenice:
Univ Nacl Autonoma Mexico, Dept Farm, Fac Quim, Ciudad Univ, Mexico City 04510, DF, Mexico
Rosas-Ramirez, Daniel:
Univ Nacl Autonoma Mexico, Inst Quim, Dept Quim Biomacromol, Ciudad Univ, Mexico City 04510, DF, Mexico
Castaneda-Gomez, Jhon:
Univ Quindio, Fac Ciencias & Tecnol, Grp Quim Invest & Desarrollo Ambiental, Armenia, Colombia
Alejandro Aparicio-Cuevas, Manuel:
Univ Nacl Autonoma Mexico, Dept Farm, Fac Quim, Ciudad Univ, Mexico City 04510, DF, Mexico
Fragoso-Serrano, Mabel:
Univ Nacl Autonoma Mexico, Dept Farm, Fac Quim, Ciudad Univ, Mexico City 04510, DF, Mexico
Figueroa-Gonzalez, Gabriela:
Univ Nacl Autonoma Mexico, Dept Farm, Fac Quim, Ciudad Univ, Mexico City 04510, DF, Mexico
Pereda-Miranda, Rogelio:
Univ Nacl Autonoma Mexico, Dept Farm, Fac Quim, Ciudad Univ, Mexico City 04510, DF, Mexico
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