Bioguided study of the in vitro parasitocidal effect on adult Hymenolepis nana of the Psidium sartorianum (O. Berg Nied.) fruit methanol extract
Por:
Montes-Avila J., Díaz-Camacho S.P., Willms K., de-la-Cruz-Otero M.C., Robert L., Rivero I.A., Delgado-Vargas F.
Publicada:
1 nov 2017
Resumen:
Intestinal parasites have a high prevalence in many developing
countries. In particular, Hymenolepis nana affects primarily children
and available treatments are not 100% effective. In Mexico, Psidium
sartorianum fruit has been used in traditional medicine to cure
intestinal worms. In this paper, the in vitro antiparasitic activity of
the methanol extract from P. sartorianum fruit was analyzed by a
bioguided strategy and through light and electronic microscopy.
Chloroform fraction (CF) of the methanol extract and its sub-fraction
(F4-sCF) showed the highest activities; the results for the times of
paralysis (5 min) and death (20 min) and the ultrastructural damage were
better with F4-sCF than those found using the commercially pure compound
Praziquantel. The compound identified in the F4-sCF by nuclear magnetic
resonance (H-1 and C-13 nuclear magnetic resonance) and
gas-chromatography mass-spectrometry was the
2',6'-dihidroxy-4'-methoxychalcone (pinostrobin chalcone). Accordingly
to the literature, this chalcone may be acting by inhibition of tubulin
polymerization and apoptosis induction, but future studies must identify
what the molecular targets in H. nana are behind the effect of this
chalcone.
Filiaciones:
Montes-Avila J.:
Autonomous Univ Sinaloa FCQB UAS, Fac Chem & Biol Sci, Ciudad Univ S-N, Culiacan 80010, SIN, Mexico
Grad Programs Biomed Sci, Ciudad Univ S-N, Culiacan 80010, SIN, Mexico
Faculty of Chemical and Biological Sciences of the Autonomous University of Sinaloa (FCQB-UAS), Ciudad Universitaria s/n, Culiacan, SIN 80010, Mexico
Graduate Programs in Biomedical Sciences, Ciudad Universitaria s/n, Culiacan, SIN 80010, Mexico
Díaz-Camacho S.P.:
Faculty of Chemical and Biological Sciences of the Autonomous University of Sinaloa (FCQB-UAS), Ciudad Universitaria s/n, Culiacan, SIN 80010, Mexico
Graduate Programs in Biomedical Sciences, Ciudad Universitaria s/n, Culiacan, SIN 80010, Mexico
Graduate Programs in Biotechnology of the FCQB-UAS, Ciudad Universitaria s/n, Culiacan, SIN 80010, Mexico
Willms K.:
Natl Autonomous Univ Mexico UNAM, Dept Microbiol & Parasitol, Fac Med, Mexico City 04510, DF, Mexico
Department of Microbiology and Parasitology, Faculty of Medicine, National Autonomous University of Mexico (UNAM), Mexico, DF 04510, Mexico
de-la-Cruz-Otero M.C.:
Autonomous Univ Sinaloa FCQB UAS, Fac Chem & Biol Sci, Ciudad Univ S-N, Culiacan 80010, SIN, Mexico
Faculty of Chemical and Biological Sciences of the Autonomous University of Sinaloa (FCQB-UAS), Ciudad Universitaria s/n, Culiacan, SIN 80010, Mexico
Robert L.:
Natl Autonomous Univ Mexico UNAM, Dept Microbiol & Parasitol, Fac Med, Mexico City 04510, DF, Mexico
Department of Microbiology and Parasitology, Faculty of Medicine, National Autonomous University of Mexico (UNAM), Mexico, DF 04510, Mexico
Rivero I.A.:
Inst Tecnol Tijuana, Ctr Grad & Invest Quim, Apartado Postal 1166, Tijuana 22000, BC, Mexico
Instituto Tecnológico de Tijuana, Centro de Graduados e Investigación en Química, Apartado Postal 1166, Tijuana, BC 22000, Mexico
Delgado-Vargas F.:
FCQB UAS, Grad Programs Biotechnol, Ciudad Univ S-N, Culiacan 80010, SIN, Mexico
FCQB UAS, Grad Program Food Sci & Technol, Ciudad Univ S-N, Culiacan 80010, SIN, Mexico
Graduate Programs in Biotechnology of the FCQB-UAS, Ciudad Universitaria s/n, Culiacan, SIN 80010, Mexico
Graduate Program in Food Science and Technology of the FCQB-UAS, Ciudad Universitaria s/n, Culiacan, SIN 80010, Mexico
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