The master regulators of the fla1 and fla2 flagella of rhodobacter sphaeroides control the expression of their cognate chey proteins
Por:
Hernandez-Valle J., Domenzain C., de la Mora J., Poggio S., Dreyfus G., Camarena L.
Publicada:
1 mar 2017
Resumen:
Rhodobacter sphaeroides is an alphaproteobacterium that has two complete sets of flagellar genes. The fla1 set was acquired by horizontal transfer from an ancestral gammaproteobacterium and is the only set of flagellar genes that is expressed during growth under standard laboratory conditions. The products of these genes assemble a single, subpolar flagellum. In the absence of the Fla1 flagellum, a gain-of-function mutation in the histidine kinase CckA turns on the expression of the fla2 flagellar genes through the response regulator CtrA. The rotation of the Fla1 and Fla2 flagella is controlled by different sets of chemotaxis proteins. Here, we show that the expression of the chemotaxis proteins that control Fla2, along with the expression of the fla2 genes, is coordinated by CtrA, whereas the expression of the chemotaxis genes that control Fla1 is mediated by the master regulators of the Fla1 system. The coordinated expression of the chemosensory proteins with their cognate flagellar genes highlights the relevance of integrating the expression of the horizontally acquired fla1 genes with a chemosensory system independently of the regulatory proteins responsible for the expression of fla2 and its cognate chemosensory system. © 2017 American Society for Microbiology. All Rights Reserved.
Filiaciones:
Hernandez-Valle J.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City, DF, Mexico
Instituto de Investigaciones Biomédicasa, Universidad Nacional Autónoma de México, Mexico City, Mexico
Domenzain C.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City, DF, Mexico
Instituto de Investigaciones Biomédicasa, Universidad Nacional Autónoma de México, Mexico City, Mexico
de la Mora J.:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City, DF, Mexico
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico
Poggio S.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City, DF, Mexico
Instituto de Investigaciones Biomédicasa, Universidad Nacional Autónoma de México, Mexico City, Mexico
Dreyfus G.:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City, DF, Mexico
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico
Camarena L.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City, DF, Mexico
Instituto de Investigaciones Biomédicasa, Universidad Nacional Autónoma de México, Mexico City, Mexico
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