1,2-Diacylglycerol choline phosphotransferase catalyzes the final step in the unique Treponema denticola phosphatidylcholine biosynthesis pathway
Por:
Angel Vences-Guzman, Miguel, Goetting-Minesky, M. Paula, Guan, Ziqiang, Castillo-Ramirez, Santiago, America Cordoba-Castro, Luz, Lopez-Lara, Isabel M., Geiger, Otto, Sohlenkamp, Christian, Fenno, J. Christopher
Publicada:
1 mar 2017
Resumen:
Treponema denticola synthesizes phosphatidylcholine through a licCA-dependent CDP-choline pathway identified only in the genus Treponema. However, the mechanism of conversion of CDP-choline to phosphatidylcholine remained unclear. We report here characterization of TDE0021 (herein designated cpt) encoding a 1,2-diacylglycerol choline phosphotransferase homologous to choline phosphotransferases that catalyze the final step of the highly conserved Kennedy pathway for phosphatidylcholine synthesis in eukaryotes. T. denticola Cpt catalyzed in vitro phosphatidylcholine formation from CDP-choline and diacylglycerol, and full activity required divalent manganese. Allelic replacement mutagenesis of cpt in T. denticola resulted in abrogation of phosphatidylcholine synthesis. T. denticola Cpt complemented a Saccharomyces cerevisiae CPT1 mutant, and expression of the entire T. denticola LicCA-Cpt pathway in E. coli resulted in phosphatidylcholine biosynthesis. Our findings show that T. denticola possesses a unique phosphatidylcholine synthesis pathway combining conserved prokaryotic choline kinase and CTP:phosphocholine cytidylyltransferase activities with a 1,2-diacylglycerol choline phosphotransferase that is common in eukaryotes. Other than in a subset of mammalian host-associated Treponema that includes T. pallidum, this pathway is found in neither bacteria nor Archaea. Molecular dating analysis of the Cpt gene family suggests that a horizontal gene transfer event introduced this gene into an ancestral Treponema well after its divergence from other spirochetes. © 2016 John Wiley & Sons Ltd
Filiaciones:
Angel Vences-Guzman, Miguel:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Ave Univ S-N,Apdo Postal 565-A, Cuernavaca 62210, Morelos, Mexico
Goetting-Minesky, M. Paula:
Univ Michigan, Sch Dent, Dept Biol & Mat Sci, Ann Arbor, MI 48109 USA
Guan, Ziqiang:
Duke Univ, Med Ctr, Dept Biochem, Durham, NC 27710 USA
Castillo-Ramirez, Santiago:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Ave Univ S-N,Apdo Postal 565-A, Cuernavaca 62210, Morelos, Mexico
America Cordoba-Castro, Luz:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Ave Univ S-N,Apdo Postal 565-A, Cuernavaca 62210, Morelos, Mexico
Lopez-Lara, Isabel M.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Ave Univ S-N,Apdo Postal 565-A, Cuernavaca 62210, Morelos, Mexico
Geiger, Otto:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Ave Univ S-N,Apdo Postal 565-A, Cuernavaca 62210, Morelos, Mexico
Sohlenkamp, Christian:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Ave Univ S-N,Apdo Postal 565-A, Cuernavaca 62210, Morelos, Mexico
Fenno, J. Christopher:
Univ Michigan, Sch Dent, Dept Biol & Mat Sci, Ann Arbor, MI 48109 USA
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