Antimicrobial and anti-inflammatory activities, wound-healing effectiveness and chemical characterization of the latex of Jatropha neopauciflora Pax
Por:
Hernandez-Hernandez A.B., Alarcon-Aguilar F.J., Almanza-Perez J.C., Nieto-Yañez O., Olivares-Sanchez J.M., Duran-Diaz A., Rodriguez-Monroy M.A., Canales-Martinez M.M.
Publicada:
23 may 2017
Resumen:
Ethnopharmacological relevance: Jatropha neopauciflora Pax is an endemic
species to Mexico, and its latex is used in traditional medicine to
treat mouth infections when there are loose teeth and to heal wounds. In
this research, we evaluated the antimicrobial activity, wound healing
efficacy and chemical characterization of J. neopauciflora latex in a
murine model.
Materials and methods: The antibacterial activity was determined using
Gram positive and negative strains, the antifungal activity was
determined using yeast and filamentous fungi, and the wound healing
efficacy of the latex was determined using the tensiometric method. The
anti-inflammatory activity was evaluated using the plantar oedema model
in rats, administering the latex orally and topically. Cytotoxic
activity was determined in vitro in two different cell lines.
Antioxidant capacity, total phenolics, total flavonoids, reducing
carbohydrates and latex proteins were quantified. The latex analysis was
performed by High Performance Liquid Chromatography (HPLC). Finally,
molecular exclusion chromatography was performed.
Results: The latex demonstrated antibacterial activity. The most
sensitive strains were Gram positive bacteria, particularly S. aureus
(MIC=2 mg/mL), and the latex had bacteriostatic activity. The latex did
not show antifungal activity. The latex demonstrated a wound-healing
efficacy, even the positive control (Recoveron). The orally administered
latex demonstrated the best anti-inflammatory activity and was not toxic
to either of the 2 cell lines. The latex had a high antioxidant capacity
(SA(50)=5.4 mu g/mL), directly related to the total phenolic (6.9 mu g
GAE/mL) and flavonoid (12.53 mu g QE/mL) concentration. The carbohydrate
concentration was 18.52 mu g/mL, and fructose was the most abundantly
expressed carbohydrate in the latex (14.63 mu g/mL, 79.03%).
Additionally, the latex contained proteins (7.62 mu g/mL) in its
chemical constitution. As secondary metabolites, the HPLC analysis
indicated the presence of phenols and flavonoids.
Conclusions: The J. neopauciflora latex promotes the wound healing
process by avoiding microorganism infections, inhibiting inflammation
and acting as an antioxidant.
Filiaciones:
Hernandez-Hernandez A.B.:
UAM I, DCBS, Posgrad Biol Expt, Mexico City, DF, Mexico
UNAM, Fac Estudios Super Iztacala, UBIPRO, Lab Farmacognosia, Edo, Mex, Mexico
Posgrado en Biologia Experimental, Division en Ciencias Biologicas y de la Salud (DCBS), Universidad Autonoma Metropolitana Iztapalapa (UAM-I), Mexico
Laboratorio de Farmacognosia, UBIPRO Facultad de Estudios Superiores Iztacala UNAM, Tlalnepantla, Edo, Mex, Mexico
Alarcon-Aguilar F.J.:
UAM I, Dept Ciencias Salud, Lab Farmacol, DCBS, Mexico City, DF, Mexico
Laboratorio de Farmacologia, Departamento de Ciencias de la Salud, DCBS, UAM-I, Mexico, D.F., Mexico
Almanza-Perez J.C.:
UAM I, Dept Ciencias Salud, Lab Farmacol, DCBS, Mexico City, DF, Mexico
Laboratorio de Farmacologia, Departamento de Ciencias de la Salud, DCBS, UAM-I, Mexico, D.F., Mexico
Nieto-Yañez O.:
UNAM, Fac Estudios Super Iztacala, Carrera Med, Edo, Mex, Mexico
Carrera de Medicina Facultad de Estudios Superiores-Iztacala UNAM, Tlalnepantla, Edo, Mex, Mexico
Olivares-Sanchez J.M.:
UNAM, Fac Estudios Super Iztacala, UBIPRO, Lab Farmacognosia, Edo, Mex, Mexico
Laboratorio de Farmacognosia, UBIPRO Facultad de Estudios Superiores Iztacala UNAM, Tlalnepantla, Edo, Mex, Mexico
Duran-Diaz A.:
UNAM, Fac Estudios Super Iztacala, UBIPRO, Lab Farmacognosia, Edo, Mex, Mexico
Laboratorio de Farmacognosia, UBIPRO Facultad de Estudios Superiores Iztacala UNAM, Tlalnepantla, Edo, Mex, Mexico
Rodriguez-Monroy M.A.:
UNAM, Fac Estudios Super Iztacala, Carrera Med, Edo, Mex, Mexico
Carrera de Medicina Facultad de Estudios Superiores-Iztacala UNAM, Tlalnepantla, Edo, Mex, Mexico
Canales-Martinez M.M.:
UNAM, Fac Estudios Super Iztacala, UBIPRO, Lab Farmacognosia, Edo, Mex, Mexico
Laboratorio de Farmacognosia, UBIPRO Facultad de Estudios Superiores Iztacala UNAM, Tlalnepantla, Edo, Mex, Mexico
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