Anxiolytic- and antidepressant-like effects of an aqueous extract of Tanacetum parthenium L. Schultz-Bip (Asteraceae) in mice
Por:
Cardenas, Jorge, Reyes-Perez, Valeria, Dolores Hernandez-Navarro, Maria, Maria Dorantes-Barron, Ana, Almazan, Salvador, Estrada-Reyes, Rosa
Publicada:
22 mar 2017
Resumen:
Aim: Tanacetum parthenium L. Schultz-Bip (Asteraceae) is widely used
worldwide in traditional medicine for the treatment of convulsions and
culture-bound syndromes such as susto (fear). The aim of this work was
to evaluate the anxiolytic- and antidepressant-like effects of an
aqueous extract of T. parthenium in behavioral paradigms in mice. The
effects of T. parthenium were compared with those produced by anxiolytic
and antidepressant drugs. We carried out the chemical characterization
of the main constituents of T. parthenium. The involvement with the
GABAergic and serotoninergic neurotransmitter systems were explored be
means of synergic and antagonist experiments.
Materials and methods: The anxiolytic-like effect was evaluated using
the Burying Behavior Test (BBT) and the Elevated Plus-Maze Test (PMT).
The antidepressant-like effect was evaluated in the Forced Swimming Test
(FST), and ambulatory activity was assessed in the Open Field Test
(OFT). Employing the behavioral tests, synergism and antagonism
experiments with Alprazolam, Muscimol, and Picrotoxin were carried out
in the PMT. In a series of independent experiments, concomitant
administration of T. parthenium and Alprazolam, Fluoxetine, or
p-chlorophenylalanine were conducted in the FST.
For chemical characterization, High-Performance Liquid
Chromatography-Electra Spray Ionization-Mass Spectrometry (HPLC-ESI-MS)
analysis was performed.
Results: T. parthenium exerts clear anxiolytic- and antidepressant-like
effects in mice, without affecting the ambulatory activity of the
experimental subjects.
Conclusions: Anxiolytic- and antidepressant-like T. parthenium effects
result, at least part from the involvement of the GABAergic system. Our
results support the use of Tanacetum parthenium in traditional medicine
and suggest its therapeutic potential in the comorbid anxiety and
depression.
Filiaciones:
Cardenas, Jorge:
Univ Nacl Autonoma Mexico, Inst Quim, Mexico City 04510, DF, Mexico
Reyes-Perez, Valeria:
Univ Nacl Autonoma Mexico, Inst Quim, Mexico City 04510, DF, Mexico
UAEM, Fac Quim, Toluca, Estado De Mexic, Mexico
INPRFM, Lab Fitofarmacol, Calzada Mexico Xochimilco 101, Tlalpan 14370, CDMX, Mexico
Dolores Hernandez-Navarro, Maria:
UAEM, Fac Quim, Toluca, Estado De Mexic, Mexico
Maria Dorantes-Barron, Ana:
INPRFM, Lab Fitofarmacol, Calzada Mexico Xochimilco 101, Tlalpan 14370, CDMX, Mexico
Almazan, Salvador:
INPRFM, Dept Bioelect, Direct Inuest Neurociencias, Calzada Mexico Xochimilco 101, Tlalpan 14370, CDMX, Mexico
Estrada-Reyes, Rosa:
INPRFM, Lab Fitofarmacol, Calzada Mexico Xochimilco 101, Tlalpan 14370, CDMX, Mexico
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