Th17 cells and neutrophils: Close collaborators in chronic Leishmania mexicana infections leading to disease severity
Por:
Pedraza-Zamora, C. P., Delgado-Dominguez, J., Zamora-Chimal, J., Becker, I.
Publicada:
1 abr 2017
Resumen:
Cutaneous leishmaniasis caused by Leishmania mexicana is associated with
an important inflammatory response. We here analysed the kinetics of
Th17 cells and neutrophils in ear lobe lesions caused by Leishmania
mexicana throughout 90 days of disease progression in susceptible BALB/c
and semi-resistant C57BL/6 mice infected with 1 x 10(5) Leishmania
mexicana promastigotes. Cells in the lesions were extracted and
quantified by flow cytometry, whereas their distribution in the tissues
in relation to the parasites was analysed by immunohistochemistry. Our
results show that in BALB/c mice, both Th17 cells and neutrophils
increase concomitantly and to significantly higher levels on day 90
post-infection, as compared to C57BL/6 mice. Our results provide novel
evidence on the cells causing chronic inflammation throughout Leishmania
mexicana infections, resulting as a consequence of neutrophil
recruitment together with Th17 cell differentiation and recruitment,
both of which remain in the infection site throughout the late phase of
the infection. We conclude that the more enhanced levels of Th17 cells
and neutrophils during chronic inflammatory lesions in BALB/c mice
participate in their enhanced susceptibility towards a
progressive-disease evolution, whereas the more controlled response of
these cells in C57BL/6 mice -possibly relates to the more resistant
profile of this mouse strain.
Filiaciones:
Pedraza-Zamora, C. P.:
Facultad de Medicina, Unidad de Investigación en Medicina Experimental, Universidad Nacional Autónoma de México (UNAM), Hospital General de México, Ciudad de México, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Unidad Invest Med Expt, Hosp Gen Mexico, Mexico City, DF, Mexico
Delgado-Dominguez, J.:
Facultad de Medicina, Unidad de Investigación en Medicina Experimental, Universidad Nacional Autónoma de México (UNAM), Hospital General de México, Ciudad de México, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Unidad Invest Med Expt, Hosp Gen Mexico, Mexico City, DF, Mexico
Zamora-Chimal, J.:
Facultad de Medicina, Unidad de Investigación en Medicina Experimental, Universidad Nacional Autónoma de México (UNAM), Hospital General de México, Ciudad de México, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Unidad Invest Med Expt, Hosp Gen Mexico, Mexico City, DF, Mexico
Becker, I.:
Facultad de Medicina, Unidad de Investigación en Medicina Experimental, Universidad Nacional Autónoma de México (UNAM), Hospital General de México, Ciudad de México, Mexico
Univ Nacl Autonoma Mexico, Fac Med, Unidad Invest Med Expt, Hosp Gen Mexico, Mexico City, DF, Mexico
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