Shock Wave- Induced Damage and Poration in Eukaryotic Cell Membranes
Por:
López-Marín, L.M., Millán-Chiu, B.E., Castaño-González, K., Aceves, C., Fernández, F., Varela-Echavarría, A., Loske, A.M.
Publicada:
1 feb 2017
Resumen:
Shock waves are known to permeabilize eukaryotic cell membranes, which
may be a powerful tool for a variety of drug delivery applications.
However, the mechanisms involved in shock wave- mediated membrane
permeabilization are still poorly understood. In this study, the effects
on both the permeability and the ultrastructural features of two human
cell lineages were investigated after the application of underwater
shock waves in vitro. Scanning Electron Microscopy of cells derived from
a human embryo kidney ( HEK)- 293 and Michigan Cancer Foundation ( MCF)-
7 cells, an immortalized culture derived from human breast
adenocarcinoma, showed a small amount of microvilli ( as compared to
control cells), the presence of hole- like structures, and a decrease in
cell size after shock wave exposure. Interestingly, these effects were
accompanied by the permeabilization of acid and macromolecular dyes and
gene transfection. Trypan blue exclusion assays indicated that cell
membranes were porated during shock wave treatment but resealed after a
few seconds. Deformations of the cell membrane lasted for at least 5
min.
Filiaciones:
López-Marín, L.M.:
Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro, Qro., Mexico
Millán-Chiu, B.E.:
CONACYT - Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro, Qro., Mexico
Castaño-González, K.:
Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro, Qro., Mexico
Aceves, C.:
Instituto de Neurobiología, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro, Qro., Mexico
Fernández, F.:
Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro, Qro., Mexico
Varela-Echavarría, A.:
Instituto de Neurobiología, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro, Qro., Mexico
Loske, A.M.:
Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, Querétaro, Qro., Mexico
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