Lipoteichoic acid of Streptococcus sanguinis induces the expression of cyclooxygenase-2 via MAPK signaling pathways in H9c2 cells
Por:
Gutierrez-Venegas, Gloria, Flores-Hernandez, Israel
Publicada:
1 ene 2017
Resumen:
Bloodstream infections, including bacteremia and infective endocarditis, represent important medical conditions resulting in high mortality rates. Streptococcus sanguinis are dental plaque Gram-positive organisms associated to infective endocarditis. Lipoteichoic acid, is a component of the outer membrane of Gram-positive bacteria and can cause septic shock. H9c2 cells were exposed to lipoteichoic acid in order to determine levels of nuclear factor (NF)-?B, mitogen activated protein kinase (MAPK) and cyclooxygenase-2. The results are presented as mean ± SE obtained from three independent experiments. A p value of < 0.05 was considered statistically significant. In this study, we characterized lipoteichoic acid signal-transduction mechanisms on H9c2 cardiomyoblasts. When H9c2 cells were exposed to lipoteichoic acid (15 µg/ml) they induced time-dependent augmented phoshorylation of MAPK; they also enhanced cytosolic and nuclear NF-?B levels in time-dependent manner. Furthermore, lipoteichoic acid significantly increased LTA-induced COX-2. LTA-mediated COX-2 expression was inhibited by PD98059, SP-600125 and calphostin C. The present study showed that lipoteichoic acid obtained from Streptococcus sanguinis can increase cyclooxygenase-2 synthesis through protein kinase C, mitogen activated protein kinases and NF-?B activation in H9c2.
Filiaciones:
Gutierrez-Venegas, Gloria:
Laboratorio de Bioquímica, División de Estudios de Posgrado e Investigación de la Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico
Univ Nacl Autonoma Mexico, Fac Odontol, Div Estudios Posgrado & Invest, Lab Bioquim, Mexico City 04510, DF, Mexico
Flores-Hernandez, Israel:
Laboratorio de Bioquímica, División de Estudios de Posgrado e Investigación de la Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico
Univ Nacl Autonoma Mexico, Fac Odontol, Div Estudios Posgrado & Invest, Lab Bioquim, Mexico City 04510, DF, Mexico
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