SynapCountJ: A tool for analyzing synaptic densities in neurons
Por:
Mata, G., Heras, J., Morales, M., Romero, A., Rubio, J.
Publicada:
1 ene 2016
Resumen:
The quantification of synapses is instrumental to measure the evolution of synaptic densities of neurons under the effect of some physiological conditions, neuronal diseases or even drug treatments. However, the manual quantification of synapses is a tedious, error-prone, time-consuming and subjective task; therefore, tools that might automate this process are desirable. In this paper, we present SynapCountJ, an ImageJ plugin, that can measure synaptic density of individual neurons obtained by immunofluorescence techniques, and also can be applied for batch processing of neurons that have been obtained in the same experiment or using the same setting. The procedure to quantify synapses implemented in SynapCountJ is based on the colocalization of three images of the same neuron (the neuron marked with two antibody markers and the structure of the neuron) and is inspired by methods coming from Computational Algebraic Topology. SynapCountJ provides a procedure to semi-automatically quantify the number of synapses of neuron cultures; as a result, the time required for such an analysis is greatly reduced. © 2016 by SCITEPRESS - Science and Technology Publications, Lda. All rights reserved.
Filiaciones:
Mata, G.:
Departamento de Mate?aticas Y Computacíon, Universidad de la Rioja, Logroño, La Rioja, Spain
Heras, J.:
Departamento de Mate?aticas Y Computacíon, Universidad de la Rioja, Logroño, La Rioja, Spain
Morales, M.:
Institut de Neurocincies, Universitat Autonoma de Barcelona, Barcelona, Spain
Romero, A.:
Departamento de Mate?aticas Y Computacíon, Universidad de la Rioja, Logroño, La Rioja, Spain
Rubio, J.:
Departamento de Mate?aticas Y Computacíon, Universidad de la Rioja, Logroño, La Rioja, Spain
Gilbert J.:
Departamento de Mate?aticas Y Computacíon, Universidad de la Rioja, Logroño, La Rioja, Spain
Fred A.:
Departamento de Mate?aticas Y Computacíon, Universidad de la Rioja, Logroño, La Rioja, Spain
Quintao C.:
Departamento de Mate?aticas Y Computacíon, Universidad de la Rioja, Logroño, La Rioja, Spain
Sliwa J.:
Departamento de Mate?aticas Y Computacíon, Universidad de la Rioja, Logroño, La Rioja, Spain
Azhari H.:
Departamento de Mate?aticas Y Computacíon, Universidad de la Rioja, Logroño, La Rioja, Spain
Ruiz C.:
Departamento de Mate?aticas Y Computacíon, Universidad de la Rioja, Logroño, La Rioja, Spain
Gamboa H.:
Departamento de Mate?aticas Y Computacíon, Universidad de la Rioja, Logroño, La Rioja, Spain
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