Evaluation of the genotoxic potential of dimethyl sulfoxide (DMSO) in meristematic cells of the root of Vicia faba
Por:
Valencia-Quintana R., Gómez-Arroyo S., Waliszewski S.M., Sánchez-Alarcón J., Gómez-Olivares J.L., Flores-Márquez A.R., Cortés-Eslava J., Villalobos-Pietrini R.
Publicada:
1 ene 2012
Resumen:
Dimethyl sulfoxide (DMSO) is an organic solvent with several biological applications. It is extensively used to dissolve compounds that hardly dissolve in water to detect their genotoxic activity in vitro. In this study DMSO will be tested to determine its genotoxic potential. The effect of DMSO on the frequency of chromosomal aberrations in anaphase as well as DNA fragmentation through the comet assay has been evaluated in the meristematic cells of the root tips of Vicia faba. It has been observed that the frequency of chromosomal aberrations increases when the concentration of DMSO increases, reaching its maximum value with 20% of DMSO and decreasing at 30 and 40% of DMSO, in comparison to this maximum value, but significantly higher than the values observed in the control. Similarly, the percentage of fragmentation and damage index evaluated through the comet assay showed the same behavior; some of the possible mechanisms of action are discussed. © 2012 Korean Society of Environmental Risk Assessment and Health Science and Springer Science+Business Media Dordrecht.
Filiaciones:
Valencia-Quintana R.:
Universidad Autónoma de Tlaxcala, Tlaxcala, Mexico
Gómez-Arroyo S.:
Universidad Nacional Autonoma de Mexico, D.F. Mexico, Mexico
Waliszewski S.M.:
Universidad Veracruzana, Veracruz, Mexico
Sánchez-Alarcón J.:
Universidad Autónoma de Tlaxcala, Tlaxcala, Mexico
Gómez-Olivares J.L.:
Universidad Autonoma Metropolitana, D.F. Mexico, Mexico
Flores-Márquez A.R.:
Universidad Nacional Autonoma de Mexico, D.F. Mexico, Mexico
Cortés-Eslava J.:
Universidad Nacional Autonoma de Mexico, D.F. Mexico, Mexico
Villalobos-Pietrini R.:
Universidad Nacional Autonoma de Mexico, D.F. Mexico, Mexico
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