Molecular responses of Escherichia coli caused by heat stress and recombinant protein production during temperature induction
Por:
Valdez-Cruz N.A., Ramírez O.T., Trujillo-Roldán M.A.
Publicada:
1 ene 2011
Resumen:
In a recent review, we discussed the extensively used temperature-inducible expression system, based on the pL and/or pR phage lambda promoters that are finely regulated by the thermolabile cI857 repressor. In this system, an increase in temperature induces the heterologous protein production and activates the heat shock response, as well as the stringent and SOS responses. The same responses are activated just by the overproduction of recombinant protein. All such responses result in a metabolicburden to the cells, a decrease in the specificgrowth rate and alterations in the central carbon metabolism. Altogether, these effects can alter the quantity and quality of the produced foreign protein. Here, we compare and discuss the transcription of selected genes, and the concomitant synthesis of heat-shock proteins (hsp) soon after thermal induction, in relation to the responses that occur in other expression systems that also trigger the heat-shock response. © 2011 Landes Bioscience.
Filiaciones:
Valdez-Cruz N.A.:
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
Ramírez O.T.:
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
Trujillo-Roldán M.A.:
Unidad de Bioprocesos, Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
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