Antigenic and genomic diversity of human rotavirus VP4 in two consecutive epidemic seasons in Mexico
Por:
Padilla-Noriega L., Méndez-Toss M., Menchaca G., Contreras J.F., Romero-Guido P., Puerto F.I., Guiscafré H., Mota F., Herrera I., Cedillo R., Muñoz O., Calva J., De Guerrero M.L., Coulson B.S., Greenberg H.B., López S., Arias C.F.
Publicada:
1 ene 1998
Resumen:
In the present investigation we characterized the antigenic diversity of the VP4 and VP7 proteins in 309 and 261 human rotavirus strains isolated during two consecutive epidemic seasons, respectively, in three different regions of Mexico. G3 was found to be the prevalent VP7 serotype during the first year, being superseded by serotype G1 strains during the second season. To antigenically characterize the VP4 protein of the strains isolated, we used five neutralizing monoclonal antibodies (MAbs) which showed specificity for VP4 serotypes P1A, P1B, and P2 in earlier studies. Eight different patterns of reactivity with these MAbs were found, and the prevalence of three of these patterns varied from one season to the next. The P genotype of a subset of 52 samples was determined by PCR. Among the strains characterized as genotype P[4] and P[8] there were three and five different VP4 MAb reactivity patterns, respectively, indicating that the diversity of neutralization epitopes in VP4 is greater than that previously appreciated by the genomic typing methods.
Filiaciones:
Padilla-Noriega L.:
Instituto de Biotecnología, Univ. Nac. Auton. de México, Cuernavaca, Morelos, Mexico
Depto. de Biología Molecular, Inst. de Invest. Biomédicas, Univ. Nac. Auton. de México, Apdo. Postal 70-228, Mexico City 04510, Mexico
Méndez-Toss M.:
Instituto de Biotecnología, Univ. Nac. Auton. de México, Cuernavaca, Morelos, Mexico
Menchaca G.:
Fac. de Ciencias Biológicas, Univ. Auton. de Nuevo León, Monterrey, Nuevo León, Mexico
Contreras J.F.:
Fac. de Ciencias Biológicas, Univ. Auton. de Nuevo León, Monterrey, Nuevo León, Mexico
Romero-Guido P.:
Instituto de Biotecnología, Univ. Nac. Auton. de México, Cuernavaca, Morelos, Mexico
Puerto F.I.:
Ctro. Invest. Regionales Hideyo N., Univ. Auton. de Yucatán, Mérida, Yucatán, Mexico
Guiscafré H.:
Instituto Mexicano del Seguro Social, Hospital Infantil de México, Mexico City, Mexico
Mota F.:
Unidad de Rehidratación Oral, Hospital Infantil de México, Mexico City, Mexico
Herrera I.:
Facultad de Medicina, Univ. Auton. de S. Luis Potosi, San Luis Potosí, Mexico
Cedillo R.:
Instituto Mexicano del Seguro Social, Hospital Infantil de México, Mexico City, Mexico
Muñoz O.:
Instituto Mexicano del Seguro Social, Hospital Infantil de México, Mexico City, Mexico
Calva J.:
Inst. Nac. la Nutr. Salvador Zubiran, Mexico, Mexico
De Guerrero M.L.:
Inst. Nac. la Nutr. Salvador Zubiran, Mexico, Mexico
Coulson B.S.:
University of Melbourne, Parkville, Vic., Australia
Greenberg H.B.:
Stanford University, Stanford, CA, United States
López S.:
Instituto de Biotecnología, Univ. Nac. Auton. de México, Cuernavaca, Morelos, Mexico
Arias C.F.:
Instituto de Biotecnología, Univ. Nac. Auton. de México, Cuernavaca, Morelos, Mexico
Inst. de Biotecnología/UNAM, A.P. 510-3, Colonia Miraval, Cuernavaca, Morelos 62250, Mexico
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