Dopamine selects glutamatergic inputs to neostriatal neurons


Por: Flores-Hernández J., Galarraga E., Bargas J.

Publicada: 1 ene 1997
Resumen:
Glutamatergic synaptic potentials induced by micromolar concentrations of the potassium conductance blocker 4-aminopyridine (4-AP) were recorded intracellularly from rat neostriatal neurons in the presence of 10 ?M bicuculline (BIC). These synaptic potentials originate from neostriatal cortical and thalamic afferents and were completely blocked by 10 ?M 6- cyano-7-nitroquinoxaline-2,3-dione (CNQX) plus 100 ?M D-2-amino-5- phosphonovaleric acid (2-APV). Their inter-event time intervals could be fitted to exponential distributions, suggesting that they are induced randomly. Their amplitude distributions had most counts around 1 mV and fewer counts with values up to 5 mV. Since input resistance of the recorded neurons is about 40 M? the amplitudes agree to quantal size measurements in mammalian central neurons. The action of a D2 agonist, quinpirole, was studied on the frequency of these events. Mean amplitude of synaptic potentials was preserved in the presence of 2-10 ?M quinpirole, but the frequency of 4-AP-induced glutamatergic synaptic potentials was reduced in 35% of cases. The effect was blocked by the D2 antagonist sulpiride (10 ?M). Input resistance, membrane potential, or firing threshold did not change during quinpirole effect, suggesting a presynaptic site of action for quinpirole in some but not all glutamatergic afferents that make contact on a single cell. The present experiments show that dopaminergic presynaptic modulation of glutamatergic transmission in the neostriatum does not affect all stimulated afferents, suggesting that it is selective towards some of them. This may control the quality and quantity of afferent flow upon neostriatal neurons.

Filiaciones:
Flores-Hernández J.:
 Inst. de Fisiología Celular, UNAM, 04510 México City DF, Mexico

Galarraga E.:
 Inst. de Fisiología Celular, UNAM, 04510 México City DF, Mexico

Bargas J.:
 Inst. de Fisiología Celular, UNAM, 04510 México City DF, Mexico

 Inst. de Fisiología Celular, UNAM, P.O. Box 70-253, 04510, México City DF, Mexico
ISSN: 08874476
Editorial
Wiley-Liss Inc., COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA, Estados Unidos America
Tipo de documento: Article
Volumen: 25 Número: 2
Páginas: 185-195
WOS Id: A1997WF21900009
ID de PubMed: 9021899