Limitations of current diagnostic procedures for the diagnosis of Taenia solium cysticercosis in rural pigs
Por:
Sciutto E., Martínez J.J., Villalobos N.M., Hernández M., José M.V., Beltrán C., Rodarte F., Flores I., Bobadilla J.R., Fragoso G., Parkhouse M.E., Harrison L.J.S., De Aluja A.S.
Publicada:
1 ene 1998
Resumen:
The aim of the present study was to evaluate diagnostic procedures for porcine cysticercosis. Sera were obtained from 32 pigs reared in commercial farms, 47 pigs before and after experimental infection, 42 carefully necropsied rural pigs and 191 slaughtered pigs from rural communities in which the presence of the Taenia solium metacestode was assessed by tongue dissection. Sera were analyzed by ELISA to detect antibodies against T. solium antigens and to detect parasite antigens. Most sera from the necropsied rural pigs were also evaluated by the Western blot method. Antigen and antibody ELISA detection assays showed high sensitivity and specificity when applied to sera from pigs reared in commercial farms. In contrast, all methods (Ag-ELISA, Ab-ELISA assays, EITB and tongue inspection) showed lower sensitivity and specificity when applied to the generally lightly infected rurally reared pigs. The probability distribution of cysts in carcasses were also determined. These results emphasize the difficulties in detecting cysticercosis in rural pigs with low levels of cyst burdens. Copyright (C) 1998 Elsevier Science B.V.
Filiaciones:
Sciutto E.:
Inst. de Invest. Biomédicas, Univ. Nac. Autonoma Mex. (UNAM), Mexico, D.F. 04510, Mexico
Martínez J.J.:
Fac. de Med. Veterinaria y Zootecnia, Univ. Nac. Autonoma Mex. (UNAM), Mexico, D.F. 04510, Mexico
Villalobos N.M.:
Fac. de Med. Veterinaria y Zootecnia, Univ. Nac. Autonoma Mex. (UNAM), Mexico, D.F. 04510, Mexico
Hernández M.:
Inst. de Invest. Biomédicas, Univ. Nac. Autonoma Mex. (UNAM), Mexico, D.F. 04510, Mexico
José M.V.:
Inst. de Invest. Biomédicas, Univ. Nac. Autonoma Mex. (UNAM), Mexico, D.F. 04510, Mexico
Beltrán C.:
Inst. de Invest. Biomédicas, Univ. Nac. Autonoma Mex. (UNAM), Mexico, D.F. 04510, Mexico
Rodarte F.:
Fac. de Med. Veterinaria y Zootecnia, Univ. Nac. Autonoma Mex. (UNAM), Mexico, D.F. 04510, Mexico
Flores I.:
Fac. de Med. Veterinaria y Zootecnia, Univ. Nac. Autonoma Mex. (UNAM), Mexico, D.F. 04510, Mexico
Bobadilla J.R.:
Inst. de Invest. Biomédicas, Univ. Nac. Autonoma Mex. (UNAM), Mexico, D.F. 04510, Mexico
Fragoso G.:
Inst. de Invest. Biomédicas, Univ. Nac. Autonoma Mex. (UNAM), Mexico, D.F. 04510, Mexico
Parkhouse M.E.:
Institute of Animal Health, Pirbright Laboratories, Ash Road, Pirbright, United Kingdom
Harrison L.J.S.:
Department of Tropical Animal Health, Ctr. for Trop. Veterinary Medicine, Univ. of Edinburgh, Easter Bush, Roslin Midlothian EH25 9RG, United Kingdom
De Aluja A.S.:
Fac. de Med. Veterinaria y Zootecnia, Univ. Nac. Autonoma Mex. (UNAM), Mexico, D.F. 04510, Mexico
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