Inhibitory effect of sex steroids on guinea-pig airway smooth muscle contractions
Por:
Perusquía M., Hernández R., Montaño L.M., Villalón C.M., Campos M.G.
Publicada:
1 ene 1997
Resumen:
We assessed the possible inhibition of airway smooth muscle contraction by progesterone and pregnanolones (5? and 5?-reduced). Progesterone and 5?-pregnanolone prevented histamine- or carbachol-induced contraction in isolated guinea-pig trachea and potency was related to their respective chemical structure; progesterone was the most potent inhibitor in a concentration dependent manner. The steroids also exhibited calcium antagonist activities in this tissue as assessed by their action on calcium entry in depolarized preparations; this event involved the immediate blockade of the extracellular calcium influx in the muscle cell membrane, indicating a nongenomic action. Classical GABA(A), antagonists did not block the progesterone response, implying no involvement of the GABA(A)-receptor complex. Our results suggest a bronchodilating effect induced by sex steroids, and probably by other related compounds, before the genomic mechanisms take place. This nongenomic action of steroids could have potential therapeutic usefulness in the treatment of asthma.
Filiaciones:
Perusquía M.:
Depto. de Biología Celular, Inst. de Invest. Biomédicas, Univ. Nac. Auton. de México, Apdo. Postal 70-492, 04511 Mexico City, Mexico
Inst. de Invest. Biomédicas, UNAM, Apartado Postal 70-492, Mexico City, C.P. 04511, Mexico
Hernández R.:
Depto. de Biología Celular, Inst. de Invest. Biomédicas, Univ. Nac. Auton. de México, Apdo. Postal 70-492, 04511 Mexico City, Mexico
Montaño L.M.:
Depto. de Farmacología, Fac. de Medicina, Tlapan 4502, 14080 Mexico City, Mexico
Inst. Nac. de Enferm. Respiratorias, Tlapan 4502, 14080 Mexico City, Mexico
Villalón C.M.:
Terapéutica Experimental, CINVESTAV, IPN, Apdo. Postal 22026, 14000 Mexico City, Mexico
Campos M.G.:
UIM-Farmacología, Ctro. Médico Nac. Siglo XXI, Apdo. Postal 73032, 03020 Mexico City, Mexico
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