Acetylcholine and tachykinins involvement in the caffeine-induced biphasic change in intracellular Ca 2+ in bovine airway smooth muscle


Por: Montaño L.M., Carbajal V., Arreola J.L., Barajas-López C., Flores-Soto E., Vargas M.H.

Publicada: 1 ene 2003
Resumen:
1. Caffeine has been widely used as a pharmacological tool to evaluate Ca 2+ release from the sarcoplasmic reticulum in isolated smooth muscle cells. However, in nervous tissue this drug also causes neurotransmitters release, which might cause additional effects when smooth muscle strips are evaluated. To assess this last possibility, simultaneous measurements of contraction and cytosolic Ca 2+ concentration (using Fura-2/AM) were carried out in bovine airway smooth muscle strips during caffeine stimulation. 2. A first stimulation (S1, n = 11) with caffeine (10mM) induced a biphasic change in cytosolic Ca 2+, which consisted of a transient Ca 2+ peak (254 ± 40 nM, X? ± SEM) followed by a plateau (92 ± 13 nM), and a transient contraction (204.72 ± 31.56 mg tension mg tissue -1). A second caffeine stimulation (S2) produced a similar response but these parameters had a different magnitude. The S2/S1 ratios for these parameters were 0.69 ± 0.02, 0.83 ± 0.06 and 1.01 ± 0.03, respectively. Addition of ?-conotoxin GVIA (1 ?M) and tetrodotoxin (3.1 ?M) before S2 significantly diminished these S2/S1 ratios (0.26 ± 0.05, 0.26 ± 0.09 and 0.64 ± 0.11, respectively, n = 5, P < 0.05), implicating the neurotransmitters release involvement in the response to caffeine. A similar effect (P < 0.01) was observed with atropine (1 ?M, n = 4), the fragment 4-11 of substance P (SP) (an SP receptor antagonist, 10 ?M, n = 5), and with both substances (n = 4). 3. We discarded a direct effect of ?-conotoxin GVIA (1 ?M) plus tetrodotoxin (3.1 ?M) or of atropine (1 ?M) plus SP fragment 4-11 on smooth muscle cells because they did not modify caffeine responses in isolated tracheal myocytes. 4. We confirmed by HPLC that caffeine increased the release of acetylcholine (from 0.43 ± 0.19 to 2.07 ± 0.56 nM mg tissue -1, P < 0.02) in bovine airway smooth muscle strips. Detection of substance P by ELISA was not statistically different after caffeine stimulation (geometric means before and after caffeine, 0.69 vs. 1.97 pgml -1 mg tissue -1, respectively, P = 0.053). 5. We concluded that acetylcholine and tachykinins release are involved in the caffeine-induced biphasic changes in cytosolic Ca 2+ concentration.

Filiaciones:
Montaño L.M.:
 Departamento de Farmacología, Facultad de Medicina, Ciudad Universitaria, CP 04510, México DF, Mexico

 Depto. de Invest. en Asma, Inst. Nac. de Enferm. Respiratorias, Tlalpan 4502, CP 14080, México DF, Mexico

 Inst. Nac. de Enferm. Respiratorias, Tlalpan 4502, CP 14080, Mexico DF, Mexico

Carbajal V.:
 Depto. de Invest. en Asma, Inst. Nac. de Enferm. Respiratorias, Tlalpan 4502, CP 14080, México DF, Mexico

 Inst. Nac. de Enferm. Respiratorias, Tlalpan 4502, CP 14080, Mexico DF, Mexico

Arreola J.L.:
 Depto. de Invest. en Asma, Inst. Nac. de Enferm. Respiratorias, Tlalpan 4502, CP 14080, México DF, Mexico

 Inst. Nac. de Enferm. Respiratorias, Tlalpan 4502, CP 14080, Mexico DF, Mexico

Barajas-López C.:
 Dept. of Anatomy and Cell Biology, Queen's University, Kingston, Ont. K7L 3N6, Canada

Flores-Soto E.:
 Depto. de Invest. en Asma, Inst. Nac. de Enferm. Respiratorias, Tlalpan 4502, CP 14080, México DF, Mexico

 Inst. Nac. de Enferm. Respiratorias, Tlalpan 4502, CP 14080, Mexico DF, Mexico

Vargas M.H.:
 Depto. de Invest. en Asma, Inst. Nac. de Enferm. Respiratorias, Tlalpan 4502, CP 14080, México DF, Mexico

 Inst. Nac. de Enferm. Respiratorias, Tlalpan 4502, CP 14080, Mexico DF, Mexico
ISSN: 00071188
Editorial
WILEY-BLACKWELL PUBLISHING, INC, COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA, Estados Unidos America
Tipo de documento: Article
Volumen: 139 Número: 6
Páginas: 1203-1211
WOS Id: 000184318100018
ID de PubMed: 12871840
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