Trypanosoma cruzi: Allelic comparisons of the actin genes and analysis of their transcripts
Por:
Cevallos A.M., López-Villaseñor I., Espinosa N., Herrera J., Hernández R.
Publicada:
1 ene 2003
Resumen:
Two allelic genomic fragments containing actin genes from Trypanosoma cruzi were isolated and characterized. One allele comprises two complete tandem repeats of a sequence encoding an actin gene. In the other, only one actin gene is found. Each one of these three gene copies encode for a complete and identical potential protein of 376 amino acids which is 93% similar with its homolog from Trypanosoma brucei. Northern hybridizations of both total and polysomal RNA from epimastigotes demonstrated the presence of an actin polyadenylated mRNA of about 1.6kb. Actin transcripts processing sites were determined by 5'- and 3'-RACE. The obtained sequence data demonstrates that actin genes from both alleles are expressed. The stability of actin mRNA was found to be similar to the one exhibited by the ribosomal protein S4 mRNA as an internal reference. A time course analysis of cultured epimastigotes showed a novel behaviour in which actin mRNA steady state concentration peaks during the transition from the logarithmic to the stationary phase of growth. © 2003 Elsevier Science (USA). All rights reserved.
Filiaciones:
Cevallos A.M.:
Depto. Biol. Molec. y Biotecnologia, Inst. de Invest. Biomédicas, Univ. Nac. Auton. de Mex., Apartado Postal 70-228, CP 04510, México, D.F., Mexico
López-Villaseñor I.:
Depto. Biol. Molec. y Biotecnologia, Inst. de Invest. Biomédicas, Univ. Nac. Auton. de Mex., Apartado Postal 70-228, CP 04510, México, D.F., Mexico
Espinosa N.:
Depto. Biol. Molec. y Biotecnologia, Inst. de Invest. Biomédicas, Univ. Nac. Auton. de Mex., Apartado Postal 70-228, CP 04510, México, D.F., Mexico
Herrera J.:
Depto. Biol. Molec. y Biotecnologia, Inst. de Invest. Biomédicas, Univ. Nac. Auton. de Mex., Apartado Postal 70-228, CP 04510, México, D.F., Mexico
Hernández R.:
Depto. Biol. Molec. y Biotecnologia, Inst. de Invest. Biomédicas, Univ. Nac. Auton. de Mex., Apartado Postal 70-228, CP 04510, México, D.F., Mexico
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