Expression of P450-aromatase in the goat placenta throughout pregnancy
Por:
Mondragon J.A., Ocadiz-Delgado R., Miranda C., Valencia J., Rosales A.M., Gariglio P., Romano M.C.
Publicada:
1 ene 2007
Resumen:
The enzyme P450-aromatase (P450-Aro) is essential for the conversion of androgens to estrogens. The objective was to study the expression and distribution of P450-Aro in goat placentae throughout pregnancy. For this purpose, we used reverse transcription-polymerase chain reaction (RT-PCR) with primers derived from the ovarian cDNA sequence found by our group. The expression of P450-Aro mRNA was first detected by in vitro RT-PCR in cotyledons at 4 months and was still present at term. Based on in situ RT-PCR, cotyledon microvilli expressed P450-Aro mRNA early in pregnancy; the signal was detected in the syncytiotrophoblast and in non-fused cytotrophoblasts inside the microvilli, but was scarce in the interstitial cells of the villous core. In the last 2 months of pregnancy (including at term), the expression of P450-Aro mRNA was still detected in the syncytiotrophoblast. However, P450-Aro was never detected in the caruncule (regardless of stage of pregnancy). In conclusion, P450-Aro was expressed in the goat placenta microvilli starting early in pregnancy; the expression and distribution of the enzyme increased throughout pregnancy and was still present at term. © 2007 Elsevier Inc. All rights reserved.
Filiaciones:
Mondragon J.A.:
Departamento de Fisiología, Biofísica y Neurociencias, CINVESTAV, Apdo postal 14-740, 07360 México, D.F., Mexico
Ocadiz-Delgado R.:
Departamento de Genética y Biología Molecular, CINVESTAV, Apdo postal 14-740, 07360 México, D.F., Mexico
Miranda C.:
Departamento de Fisiología, Biofísica y Neurociencias, CINVESTAV, Apdo postal 14-740, 07360 México, D.F., Mexico
Valencia J.:
Departamento de Reproducción, Fac. Medicina Veterinaria y Zootecnia, UNAM, CP04510 México, D.F., Mexico
Rosales A.M.:
Departamento de Producción Agrícola y Animal, UAM-Xochimilco, Calzada del Hueso 1100, Col. Villa Quietud, Coyoacan 04960, Mexico, D.F., Mexico
Gariglio P.:
Departamento de Genética y Biología Molecular, CINVESTAV, Apdo postal 14-740, 07360 México, D.F., Mexico
Romano M.C.:
Departamento de Fisiología, Biofísica y Neurociencias, CINVESTAV, Apdo postal 14-740, 07360 México, D.F., Mexico
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