GABA (A) receptor subunits RNA expression in mice peritoneal macrophages modulate their IL-6/IL-12 production
Por:
Reyes-García M.G., Hernández-Hernández F., Hernández-Téllez B., García-Tamayo F.
Publicada:
1 ene 2007
Resumen:
The expression and functionality of the gamma aminobutyric acid A receptor (GABAAR) in mice macrophages was explored. Reverse Transcriptase-Polymerase Chain Reaction showed that macrophages express the GABAAR RNA for the ?1, ?2, ?3 and ? subunits, but not for the ?5, ?1, ?2 and ?3 subunits. The expression of ?1 subunit was also revealed by confocal microscopy. LPS-stimulated macrophages significantly reduced their in vitro production of IL-6 and IL-12 after GABA adding to the culture medium. These results showed that BALB/c mice peritoneal macrophages express a functional subset of GABAAR subunits. © 2007 Elsevier B.V. All rights reserved.
Filiaciones:
Reyes-García M.G.:
Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, México D.F. 04510, Mexico
Hernández-Hernández F.:
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México D.F. 04510, Mexico
Hernández-Téllez B.:
Departamento de Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México, México D.F. 04510, Mexico
García-Tamayo F.:
Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, México D.F. 04510, Mexico
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