Super-oxidized solution inhibits IgE-antigen-induced degranulation and cytokine release in mast cells
Por:
Medina-Tamayo J., Sánchez-Miranda E., Balleza-Tapia H., Ambriz X., Cid M.E., González-Espinosa D., Gutiérrez A.A., González-Espinosa C.
Publicada:
1 ene 2007
Resumen:
Activation of the high affinity IgE receptor (Fc?RI) through IgE-antigen complexes induces mast cell degranulation, synthesis of lipid mediators and cytokine production. These effects are involved in Type I hypersensitivity reactions and controlling them has been the main objective of many anti-allergic therapies. Here we report that pretreatment of murine bone marrow derived mast cells (BMMC) with super-oxidized solution (SOS) inhibits Fc?RI dependent-? hexosaminidase and cytokine release. This effect is exerted without altering total protein tyrosine phosphorylation, MAPK activation, cytokine mRNA accumulation or calcium mobilization after Fc?RI triggering. Our data suggest that this neutral pH-SOS acts like a mast cell-membrane stabilizer inhibiting the cell machinery for granule secretion without altering the signal transduction pathways induced by IgE-antigen receptor crosslinking. © 2007 Elsevier B.V. All rights reserved.
Filiaciones:
Medina-Tamayo J.:
Pharmacobiology Department, Cinvestav, South Campus, Mexico City, Mexico
Sánchez-Miranda E.:
Pharmacobiology Department, Cinvestav, South Campus, Mexico City, Mexico
Balleza-Tapia H.:
Pharmacobiology Department, Cinvestav, South Campus, Mexico City, Mexico
Ambriz X.:
Pharmacobiology Department, Cinvestav, South Campus, Mexico City, Mexico
Cid M.E.:
Pharmacobiology Department, Cinvestav, South Campus, Mexico City, Mexico
González-Espinosa D.:
Cell Therapy Unit, National Institute of Rehabilitation, Mexico City, Mexico
Gutiérrez A.A.:
Cell Therapy Unit, National Institute of Rehabilitation, Mexico City, Mexico
González-Espinosa C.:
Pharmacobiology Department, Cinvestav, South Campus, Mexico City, Mexico
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