Isolation of lipoxygenase isoforms from Glycine max embryo axes based on apparent cross-reactivity with anti-myosin antibodies
Por:
Islas-Flores I., Corrales-Villamar S., Bearer E., Raya J.C., Villanueva M.A.
Publicada:
1 ene 2002
Resumen:
Three lipoxygenase isoforms were isolated from Glycine max embryo axes. A number of proteins around 97 kDa cross-reacted with several anti-actin and anti-myosin antibodies and these were used to follow their purification through gel filtration, hydroxyapatite and anion exchange columns. The 97-kDa cross-reactive material eluted in the unbound fractions of the last anion exchange column, and displayed two components of pI's 6.2 and 6.3. Further phase partition of this fraction in TX-114 yielded a hydrophobic 97 kDa protein. Additionally, a 95-kDa protein was retained and eluted from this last column. Partial peptide sequences indicated that the 95 kDa protein was soybean lipoxygenase-1, the first 97 kDa protein was lypoxygenase-3, and the hydrophobic 97 kDa protein was lipoxygenase-2. Several possible reasons for the cross-reactivity with the antibodies are discussed. To our knowledge, this is the first example of individual lipoxygenase isoforms isolated from soybean embryo axes. © 2002 Elsevier Science B.V. All rights reserved.
Filiaciones:
Islas-Flores I.:
Departamento De Biología Molecular De Plantas, Instituto De Biotecnología, U.N.A.M., Apartado Postal 510-3, Cuernavaca, Morelos 62250, Mexico
Centro De Investigación Científica De Yucatán, A.C., C. 43 No. 130, Col. Chub. de Hidalgo, Mérida, Yucatán 97200, Mexico
Corrales-Villamar S.:
Departamento De Biología Molecular De Plantas, Instituto De Biotecnología, U.N.A.M., Apartado Postal 510-3, Cuernavaca, Morelos 62250, Mexico
Bearer E.:
Department of Pathology and Laboratory Medicine, Brown University, Providence, RI 02912, United States
Raya J.C.:
Departamento De Biología Molecular De Plantas, Instituto De Biotecnología, U.N.A.M., Apartado Postal 510-3, Cuernavaca, Morelos 62250, Mexico
Villanueva M.A.:
Departamento De Biología Molecular De Plantas, Instituto De Biotecnología, U.N.A.M., Apartado Postal 510-3, Cuernavaca, Morelos 62250, Mexico
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