Antimicrobial and general toxicity activities of Gymnosperma glutinosum: A comparative study
Por:
Canales M., Hernández T., Serrano R., Hernández L.B., Duran A., Ríos V., Sigrist S., Hernández H.L.H., Garcia A.M., Angeles-López O., Fernández-Araiza M.A., Avila G.
Publicada:
1 ene 2007
Resumen:
Gymnosperma glutinosum (Spreng.) Less (Asteraceae) is an important, and an effective herbal medicine which is wide used for the treatment of diarrhoea in Mexico. We examined and compared the antibacterial and antifungal activities through the dilution method and for general toxicity activity by the brine shrimp lethality assay of two samples of Gymnosperma glutinosum from two localities of Mexico: San Rafael-Coxcatlan (Puebla State) and Tepeji del Rio (Hidalgo State). In addition, two bioactive compounds (-)-17-hydroxy-neo-clerod-3-en-15-oic acid (1) and 5,7-dihydroxy-3,6,8,2?,4?,5?-hexamethoxyflavone (2) were isolated. From the hexane extract from both places was obtained a MeOH partition M2. M2 of Tepeji del Rio showed the least MICs (<125 ?g/ml) in the majority of the bacterial strains. Sarcina lutea was the most sensitive bacteria (MIC< 125 ?g/ml). The hexane extract of both localities showed antifungal activity against all tested fungi. San Rafael's hexane extract was significant more activity than Tepeji del Rio. Aspergillus niger (IC50 = 23.79 ?g/ml) and Trichophyton mentagrophytes (IC50 = 90.25 ?g/ml) were the more sensitive fungus strains. The strongest general toxicity activity was observed with the M2 partition from Tepeji del Rio (LC50 = 503.7 ?g/ml). The results obtained in this investigation, showed differences between the antimicrobial activities of the samples of plants collected in San Rafael (Puebla) and Tepeji del Rio (Hidalgo). © 2006 Elsevier Ireland Ltd. All rights reserved.
Filiaciones:
Canales M.:
Laboratorio de Fitoquímica, UBIPRO, Facultad de Estudios Superiores-Iztacala UNAM, Tlalnepantla 54090, Edo. Méx., Mexico
Hernández T.:
Laboratorio de Fitoquímica, UBIPRO, Facultad de Estudios Superiores-Iztacala UNAM, Tlalnepantla 54090, Edo. Méx., Mexico
Serrano R.:
Laboratorio de Fitoquímica, UBIPRO, Facultad de Estudios Superiores-Iztacala UNAM, Tlalnepantla 54090, Edo. Méx., Mexico
Hernández L.B.:
Laboratorio de Fitoquímica, UBIPRO, Facultad de Estudios Superiores-Iztacala UNAM, Tlalnepantla 54090, Edo. Méx., Mexico
Duran A.:
Laboratorio de Fitoquímica, UBIPRO, Facultad de Estudios Superiores-Iztacala UNAM, Tlalnepantla 54090, Edo. Méx., Mexico
Ríos V.:
Laboratorio de Fitoquímica, UBIPRO, Facultad de Estudios Superiores-Iztacala UNAM, Tlalnepantla 54090, Edo. Méx., Mexico
Sigrist S.:
Laboratorio de Fitoquímica, UBIPRO, Facultad de Estudios Superiores-Iztacala UNAM, Tlalnepantla 54090, Edo. Méx., Mexico
Hernández H.L.H.:
Acuario, Facultad de Estudios Superiores-Iztacala, UNAM, Tlalnepantla 54090, Edo. Méx., Mexico
Garcia A.M.:
Laboratorio de Fitoquímica, UBIPRO, Facultad de Estudios Superiores-Iztacala UNAM, Tlalnepantla 54090, Edo. Méx., Mexico
Angeles-López O.:
Acuario, Facultad de Estudios Superiores-Iztacala, UNAM, Tlalnepantla 54090, Edo. Méx., Mexico
Fernández-Araiza M.A.:
Acuario, Facultad de Estudios Superiores-Iztacala, UNAM, Tlalnepantla 54090, Edo. Méx., Mexico
Avila G.:
Laboratorio de Fitoquímica, UBIPRO, Facultad de Estudios Superiores-Iztacala UNAM, Tlalnepantla 54090, Edo. Méx., Mexico
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