Genetic program of neuronal differentiation and growth induced by specific activation of NMDA receptors
Por:
Ghiani C.A., Beltran-Parrazal L., Sforza D.M., Malvar J.S., Seksenyan A., Cole R., Smith D.J., Charles A., Ferchmin P.A., De Vellis J.
Publicada:
1 ene 2007
Resumen:
Glutamate and its receptors are expressed very early during development and may play important roles in neurogenesis, synapse formation and brain wiring. The levels of glutamate and activity of its receptors can be influenced by exogenous factors, leading to neurodevelopmental disorders. To investigate the role of NMDA receptors on gene regulation in a neuronal model, we used primary neuronal cultures developed from embryonic rat cerebri in serum-free medium. Using Affymetrix Gene Arrays, we found that genes known to be involved in neuronal plasticity were differentially expressed 24 h after a brief activation of NMDA receptors. The upregulation of these genes was accompanied by a sustained induction of CREB phosphorylation, and an increase in synaptophysin immunoreactivity. We conclude that NMDA receptor activation elicits expression of genes whose downstream products are involved in the regulation of early phases of the process leading to synaptogenesis and its consolidation, at least in part through sustained CREB phosphorylation. © 2006 Springer Science+Business Media, LLC.
Filiaciones:
Ghiani C.A.:
Departments of Neurobiology and Psychiatry, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, United States
Beltran-Parrazal L.:
Department of Neurology, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, United States
Sforza D.M.:
Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90024, United States
Department of Neurology, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095-7334, United States
Malvar J.S.:
Departments of Neurobiology and Psychiatry, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, United States
Seksenyan A.:
Departments of Neurobiology and Psychiatry, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, United States
Cole R.:
Departments of Neurobiology and Psychiatry, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, United States
Smith D.J.:
Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90024, United States
Charles A.:
Department of Neurology, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, United States
Ferchmin P.A.:
Department of Biochemistry, Universidad Central del Caribe, Bayamón, 00960, Puerto Rico
De Vellis J.:
Departments of Neurobiology and Psychiatry, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, United States
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