HUVECs from newborns with a strong family history of diabetes show diminished ROS synthesis in the presence of high glucose concentrations
Por:
Alvarado-Vásquez N., Páez A., Zapata E., Alcázar-Leyva S., Zenteno E., Massó F., Montaño L.F.
Publicada:
1 ene 2007
Resumen:
Background: A family history of type 2 diabetes mellitus (DM) increases the probability to develop DM and endothelial dysfunction. The probable mechanism involves augmented reactive oxygen species (ROS) synthesis. The aim of this study was to evaluate the synthesis of ROS in human umbilical vein endothelial cells (HUVECs) obtained from healthy newborns with (experimental) and without (control) a strong family history of type 2 DM, exposed to different glucose concentrations. Methods: HUVECs were exposed to various glucose concentrations for 24 and 48 h periods, before cell proliferation, mitochondrial activity, and mitochondrial membrane potential were determined. Intracellular ROS synthesis in the presence or absence of the mitochondrial uncoupler CCCP, cytochalasin B, or diphenyleneiodonium (DPI) was also evaluated. Results: As opposed to control HUVECs, we found that experimental HUVECs exposed to 30 mmol/L glucose showed a 50% decrease in cell proliferation, a 90% reduction in mitochondrial activity, and a statistically significant inhibition of ROS synthesis in the presence of CCCP or cytochalasin B; DPI had no effect. Conclusions: Our results suggest that mitochondria and NAD(P)H-oxidase from HUVECs obtained from healthy newborns with a family history of DM have an innate deficient response to high glucose concentrations. Copyright © 2006 John Wiley & Sons, Ltd.
Filiaciones:
Alvarado-Vásquez N.:
Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, Mexico
Páez A.:
Departamento de Biología Celular, Instituto Nacional de Cardiología 'Ignacio 'Cahávez', Mexico
Zapata E.:
Departamento de Biología Celular, Instituto Nacional de Cardiología 'Ignacio 'Cahávez', Mexico
Alcázar-Leyva S.:
Instituto de Investigaciones Científicas Hans-Selye, A.C., 76 000 Querétaro, Qro., Mexico
Zenteno E.:
Laboratorio de Inmunología, Departamento de Bioguímica, Facultad de Medicina UNAM, Mexico
Massó F.:
Departamento de Biología Celular, Instituto Nacional de Cardiología 'Ignacio 'Cahávez', Mexico
Montaño L.F.:
Laboratorio de Inmunología, Departamento de Bioguímica, Facultad de Medicina UNAM, Mexico
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