Visualization of the transport of primary and secondary bile acids across liver tissue in rats: In vivo study with fluorescent bile acids
Por:
Milkiewicz P., Mills C.O., Hubscher S.G., Cardenas R., Cardenas T., Williams A., Elias E.
Publicada:
1 ene 2001
Resumen:
Background/Aims: Lysyl fluorescein conjugated bile acid analogues (LFCBAA) closely parallel their natural counterparts. To assess LFCBAA as a tool for the visualization of bile acid transport within liver tissue. Methods: Wistar rats were administered playsiological concentrations of the primary bile acid analogue cholyllysyl fluoroscein (CLF) and of the secondary bile acid analogue lithocholyllysyl fluorescein (LLF) and serial liver biopsies were taken at fixed intervals. Both compounds were also injected retrogradely into the biliary tree. Frozen sections were examined by fluorescence microscopy. Results: Both CLF and LLF were rapidly taken up from sinusoidal blood but differed significantly in their hepatic handling. CLF was rapidly transported into bile, whereas LLF transport was slower and produced significantly more bile duct fluorescence. LLF clearance showed a lobular gradient with last remaining bile acid being confined largely to zone 3. Both compounds were avidly taken up by cholangiocytes after injection intravenously or retrogradely into the biliary tree. Conclusions: Visualization of LFCBAA by fluorescence microscopy may yield further information reganting hepatobiliary bile acid localization during studies of physiological and pathological mechanisms involved in transport of bile acids. The presence of both compounds within cholangiocytes strongly suggests that they may undergo a degree of chole-hepatic recirculation. © 2001 European Association for the Study of the Liver.
Filiaciones:
Milkiewicz P.:
Liver and Hepatobiliary Unit, Liver Research Laboratories, Queen Elizabeth Hospital, Edgbaston, Birmingham B15 2TH, United Kingdom
Mills C.O.:
Liver and Hepatobiliary Unit, Liver Research Laboratories, Queen Elizabeth Hospital, Edgbaston, Birmingham B15 2TH, United Kingdom
Hubscher S.G.:
Department of Pathology, University Hospital, Birmingham, United Kingdom
Cardenas R.:
Faculty of Sciences, National University of Mexico, Mexico
Cardenas T.:
Liver and Hepatobiliary Unit, Liver Research Laboratories, Queen Elizabeth Hospital, Edgbaston, Birmingham B15 2TH, United Kingdom
Williams A.:
Liver and Hepatobiliary Unit, Liver Research Laboratories, Queen Elizabeth Hospital, Edgbaston, Birmingham B15 2TH, United Kingdom
Elias E.:
Liver and Hepatobiliary Unit, Liver Research Laboratories, Queen Elizabeth Hospital, Edgbaston, Birmingham B15 2TH, United Kingdom
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