Cloning and characterization of rat casein kinase 1e


Por: Takano A., Shimizu K., Kani S., Buijs R.M., Okada M., Nagai K.

Publicada: 1 ene 2000
Resumen:
Genes differentially expressed in the subjective day and night in the rat suprachiasmatic nucleus (SCN) were surveyed by differential display. A gene homologous to human casein kinase 1? (CK1?) was isolated, which initially appeared to be expressed in the suprachiasmatic nucleus (SCN) in a circadian manner. We here describe the cDNA cloning of the rat CK1? and characterization of the protein products. The rCK1? is predominantly expressed in the brain including the SCN, binds and phosphorylates mPer1, mPer2, and mPer3 in vitro, and translocates mPer1 and mPer3, but not mPer2, to the cell nucleus depending on its kinase activity when coexpressed with these Per proteins in COS-7 cells. Copyright (C) 2000 Federation of European Biochemical Societies.

Filiaciones:
Takano A.:
 Division of Protein Metabolism, Inst. Protein Res., Osaka U., Osaka, Japan

Shimizu K.:
 Division of Protein Metabolism, Inst. Protein Res., Osaka U., Osaka, Japan

Kani S.:
 Department of Biochemistry, Kobe Univ. Sch. of Med., 650-0017, Kobe, Japan

Buijs R.M.:
 Netherlands Inst. for Brain Research, Meibergdreef 33, 1105 AZ, Amsterdam, Netherlands

Okada M.:
 Division of Protein Metabolism, Inst. Protein Res., Osaka U., Osaka, Japan

Nagai K.:
 Division of Protein Metabolism, Inst. Protein Res., Osaka U., Osaka, Japan
ISSN: 00145793
Editorial
Elsevier, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, Estados Unidos America
Tipo de documento: Article
Volumen: 477 Número: 1-2
Páginas: 106-112
WOS Id: 000088335200019
ID de PubMed: 10899319

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