A novel toxin from the venom of the scorpion Tityus trivittatus, is the first member of a new a-KTX subfamily
Por:
Abdel-Mottaleb Y., Coronas F.V., De Roodt A.R., Possani L.D., Tytgat J.
Publicada:
1 ene 2006
Resumen:
The first example of a new sub-family of toxins (?-KTx20.1) from the scorpion Tityus trivittatus was purified, sequenced and characterized physiologically. It has 29 amino acid residues, three disulfide bridges assumed to adopt the cysteine-stabilized ?/? scaffold with a pI value of 8.98. The sequence identities with all the other known ?-KTx are less than 40%. Its effects were verified using seven different cloned K+ channels (vertebrate Kv1.1-1.5, Shaker IR and hERG) expressed in Xenopus leavis oocytes. The toxin-induced effects show large differences among the different K+ channels and a preference towards Kv1.3 (EC50 = 7.9 ± 1.4 nM). © 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Filiaciones:
Abdel-Mottaleb Y.:
Laboratory of Toxicology, University of Leuven, Onderwijs and Navorsing II, Herestraat 49, Postbus 922, 3000 Leuven, Belgium
Coronas F.V.:
Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Mexico, Av. Universidad, 2001, Cuernavaca 66210, Mexico
De Roodt A.R.:
National Institute for the Production of Biologicals, Administración Nacional de Laboratorios, Instituto de Salud Dr. Carlos G. Malbran, Buenos Aires, Argentina
Possani L.D.:
Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Mexico, Av. Universidad, 2001, Cuernavaca 66210, Mexico
Tytgat J.:
Laboratory of Toxicology, University of Leuven, Onderwijs and Navorsing II, Herestraat 49, Postbus 922, 3000 Leuven, Belgium
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