Rapid determination of dry weight in human dental pulp by a colorimetric reaction
Por:
Méndez J.D., Zarzoza E.
Publicada:
1 ene 1999
Resumen:
This study was designed to assess total carbohydrate (TC) concentration, as well as the noncollagenous protein content, in human dental pulp. Pulps were obtained from eight premolars (13.10 ± 4.33 mg weight, mean ± SD) and homogenized in saline solution. TC content was as follows: 16.68 ± 9.49 ?g/mg of tissue (mean ± SD); 3.22 ± 1.69 ng of TC/mg protein (mean ± SD); and 16.23 ± 6.80 ?g of TC/mg total organic material (mean ± SD). The high concentration of carbohydrates observed in the pulp is a result of the presence of glycoproteins, glycosaminoglycans (chondroitin-6-sulfate, heparan sulfate, hyaluronic acid, and dermatan sulfate), and proteoglycans, whose structural, biochemical, and physiological functions have been well documented. Dry weight determination using dichromate solution was used because it shows less dispersion than when the data on carbohydrates and proteins are expressed as wet weight. Copyright © 1999 by The American Association of Endodontists.
Filiaciones:
Méndez J.D.:
Medical Research Unit in Metabolic Diseases, National Medical Center, Mexican Institute of Social Security, México, Mexico
Biochemistry of Diseases Laboratory, School of Odontology, National University of Mexico, México, Mexico
Medical Research Unit in Metabolic Diseases, Mexican Institute of Social Security, P.O. Box 73-032, México 03020, D.F., Mexico
Zarzoza E.:
Medical Research Unit in Metabolic Diseases, National Medical Center, Mexican Institute of Social Security, México, Mexico
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