Production of the sedative triterpene galphimine b in Galphima glauca tissue culture
Por:
Osuna L., Pereda-Miranda R., Tortoriello J., Villarreal M.L.
Publicada:
1 ene 1999
Resumen:
A tissue culture method is described for callus formation from Galphimia glauca (Mapighiaceae) in MS (Murashige and Skoog) medium supplemented with various growth regulators. Best induction was achieved when using hypocotyls as explants in medium supplemented with 2 mg/l of 2,4-dichlorophenoxyacetic acid (2,4-D). Major cellular growth of calluses was obtained with naphthaleneacetic acid (2 mg/l) + kinetin (1 mg/l). Subcultivation of calluses using various concentrations of 2,4-D allowed the production of the sedative nor-seco-triterpenoid, galphimine B and a new related compound. The structure of the new constituent was elucidated as 6-acetoxygalphimine B. The highest accumulation of active constituent 1 (1.5 x 10-2 dry weight) was achieved when 4 mg/l of the hormone were used, and this experimental condition allowed the detection of only galphimine B. A preliminary screening of the methanolic extracts prepared from calluses, using the isolated guinea- pig ileum as a general test system for pharmacological effects, demonstrated that the most active material was the one with the highest concentration of galphimine B. Total accumulation of this sedative triterpene, in the optimized tissue culture conditions, was in the same order of magnitude as the value quantified for wild plants (4.5 x 10-2 dry weight).
Filiaciones:
Osuna L.:
Ctro. de Invest. Bomedica del Sur, Instituto Mexicano del Seguro Social, Xochitepec, Argentina No. 1, Col. Centro, Morelos 62790, Mexico
Pereda-Miranda R.:
Departamento de Farmacia, Facultad de Quimica, Univ. Nacional Autonoma de Mexico, México, Mexico
Tortoriello J.:
Ctro. de Invest. Bomedica del Sur, Instituto Mexicano del Seguro Social, Xochitepec, Argentina No. 1, Col. Centro, Morelos 62790, Mexico
Villarreal M.L.:
Ctro. de Invest. Bomedica del Sur, Instituto Mexicano del Seguro Social, Xochitepec, Argentina No. 1, Col. Centro, Morelos 62790, Mexico
Centro de Invest. en Biotecnologia, Univ. Autonoma del Estado de Morelos, Cuernavaca, Morelos, Mexico
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