NeuAca2,3Gal-glycoconjugate expression determines cell susceptibility to the porcine rubulavirus LPMV
Por:
Reyes-Leyva J., Espinosa B., Hernandez J., Zenteno R., Vallejo V., Hernández-Jáuregui P., Zenteno E.
Publicada:
1 ene 1997
Resumen:
Relevance of membrane sialoglycoconjugates as receptors for infection by the porcine rubulavirus has been determined in vitro by sugar and lectin competition assays and by inhibition of glycosylation. Our results show that NeuAc?2,3Gal but not NeuAc?2,6Gal inhibits the virus infectivity of Vero cells, and the virus was effectively blocked with the lectin Maackia amurensis, specific for NeuAc?2,3Gal. Inhibition of the cellular glycosylation with tunicamycin, deoxinojirimycin as well as neuraminidase treatment diminishes the viral capacity to bind and infect this cell line. Dexamethasone, which promotes the activity of sialyl ?2,6 glycosyltransferase also diminishes the cell susceptibility for infection. This is the first report confirming that NeuAc?-2,3Gal recognition is determinant in the pathogenesis of the porcine rubulavirus.
Filiaciones:
Reyes-Leyva J.:
Laboratorio de Virología, Ctro. Invest. Biomedica de Oriente, Instituto Mexicano del Seguro Social, 2 Norte 2004, Puebla, 24010 México, Mexico
Espinosa B.:
Departamento de Bioquímica, Inst. Nac. de Enferm. Respiratorias, Tlalpan D.F., 01470 México, Mexico
Hernandez J.:
Departamento de Bioquímica, Inst. Nac. de Enferm. Respiratorias, Tlalpan D.F., 01470 México, Mexico
Zenteno R.:
Departamento de Bioquímica, Inst. Nac. de Enferm. Respiratorias, Tlalpan D.F., 01470 México, Mexico
Vallejo V.:
Laboratorio de Virología, Ctro. Invest. Biomedica de Oriente, Instituto Mexicano del Seguro Social, 2 Norte 2004, Puebla, 24010 México, Mexico
Hernández-Jáuregui P.:
Laboratorio de Virología, Ctro. Invest. Biomedica de Oriente, Instituto Mexicano del Seguro Social, 2 Norte 2004, Puebla, 24010 México, Mexico
Zenteno E.:
Laboratorio de Inmunología, Facultad de Medicina, UNAM, P.O. Box 70159, 04510 México, Mexico
Depto. Bioquímica, Fac. Medicina, UNAM, P.O. Box 70159, 04510 México, Mexico
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