Purification of the lipophosphoglycan from two Leishmania mexicana strains by lectin affinity chromatography
Por:
Gorocica P., Monroy A., Rivas B., Estrada-Parra S., Leroy Y., Chávez R., Zenteno E.
Publicada:
1 ene 1997
Resumen:
Two lipophosphoglycans (LPG) from two Leishmania mexicana (Soberano and Yucatan) strains, isolated in Mexico, were purified by affinity chromatography with the Con A lectin. The LPG from each strain, with a 10 kDa molecular weight, possesses two fractions: one with high mannose concentrations and the other with a more heterogeneous saccharidic composition; the mannose-rich fraction and the heterogeneous fraction from the Yucatan strain show a single band of identity with rabbit IgG against promastigotes from L. mexicana Yucatan. Antigen recognition was abolished by treating the high mannose LPGs with ?-mannosidase. The presence of non reductor ?-mannose sequences, as determinant epitopes in L. mexicana Soberano and Yucatan strains, was determined by mass spectrometry analysis and enzymatic cleavage.
Filiaciones:
Gorocica P.:
Departamento de Bioquímica, Inst. Nac. de Enferm. Respiratorias, México, D.F., Mexico
Ctro. de Investigaciones de Oriente, IMSS, Puebla, Mexico
Monroy A.:
Departamento de Inmunología, Esc. Nac. de Cie. Biológicas, Inst. Politécnico Nacional, México, D.F., Mexico
Rivas B.:
Departamento de Inmunología, Esc. Nac. de Cie. Biológicas, Inst. Politécnico Nacional, México, D.F., Mexico
Estrada-Parra S.:
Departamento de Inmunología, Esc. Nac. de Cie. Biológicas, Inst. Politécnico Nacional, México, D.F., Mexico
Leroy Y.:
Laboratoire de Chimie Biologique, UMR 111, Univ. des Sci. et Technol. de Lille, Villeneuve d'Ascq, France
Chávez R.:
Laboratorio de Inmunología, Departamento de Bioquímica, Facultad de Medicina UNAM, México D.F. 04510, Mexico
Zenteno E.:
Laboratorio de Inmunología, Departamento de Bioquímica, Facultad de Medicina UNAM, México D.F. 04510, Mexico
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