Effects of carbon dioxide/bicarbonate on induction of DNA single-strand breaks and formation of 8-nitroguanine, 8-oxoguanine and base-propenal mediated by peroxynitrite
Por:
Yermilov V., Yoshie Y., Rubio J., Ohshima H.
Publicada:
1 ene 1996
Resumen:
Carbon dioxide has been reported to react with peroxynitrite (ONOO-), a strong oxidant and nitrating agent, to form an ONO2CO2 - adduct, altering the reactivity characteristic of peroxynitrite. We found that bicarbonate (0-10 mM) caused a dose-dependent increase of up to 6-fold in the formation of 8-nitroguanine in calf-thymus DNA incubated with 0.1 mM peroxynitrite, whereas it produced no apparent effect on 8-oxoguanine formation. In contrast, bicarbonate inhibited peroxynitrite-induced strand breakage in plasmid pBR322 DNA and thymine-propenal formation from thymidine. We conclude that CO2/HCO3 - reacts with peroxynitrite to form a potent nitrating agent, but also to inactivate hydroxyl-radical-like activity of peroxynitrous acid.
Filiaciones:
Yermilov V.:
U. of Endogenous Cancer Risk Factors, Intl. Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon Cedex 08, France
IARC, N.N. Petrov Research Institute, Leningradskaya St. 68, Pesochny-2, 189646 St Petersburg, Russian Federation
Yoshie Y.:
U. of Endogenous Cancer Risk Factors, Intl. Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon Cedex 08, France
IARC, Tokyo University of Fisheries, 4-5-7 Konan, Minatoku, Tokyo 108, Japan
Rubio J.:
U. of Endogenous Cancer Risk Factors, Intl. Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon Cedex 08, France
Inst. de Invest. Biomédicas, Departmento de Genetica y T., CD Universitaria, Aptado Postal 70228, 04150 México DF, Mexico
Ohshima H.:
U. of Endogenous Cancer Risk Factors, Intl. Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon Cedex 08, France
Bronze
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