Cellular immune response to fractionated avian antigens by peripheral blood mononuclear cells from patients with pigeon breeder's disease
Por:
Mendoza F., Melendro E.I., Baltazares M., Banales J.L., Ximénez C., Chapela R., Selman M.
Publicada:
1 ene 1996
Resumen:
Pigeon breeder's disease (PBD), a form of hypersensitivity pneumonitis caused by repeated inhalation of antigens of pigeon origin, is characterized by a diffuse inflammation of the lower respiratory tract. Although a variety of immunologic and nonimmunologic mechanisms have been described in the development of the disease, the pathogenesis is still far from clear. In this study we analyzed the T-lymphocyte proliferative response to a variety of avion antigens with use of peripheral blood mononuclear cells from 11 patients who had PBD and 10 healthy volunteers. We used a new method based on avian antigen-bearing nitrocellulose particles derived from Western blots to study the T-cell proliferative response to 15 antigenic fractions obtained from pigeon serum. With this technique, complex mixtures of antigens can be fractionated by polyacrylamide gel electrophoresis, transferred to nitrocellulose membranes, and used for T-cell proliferation assays with selected antigenic determinants. A wide variety of responses were observed, and there were no reproducible patterns of reaction within either group. Nine of 10 healthy subjects responded to some soluble fractions. However, patients with PBD displayed the strongest response and responded to a significantly greater number of antigenic fractions. Fraction 2, representing a 220 kd molecular weight protein, was the only immunodominant antigen when both groups were compared; it was recognized by 73% of the patients with PBD and by only 20% of control subjects (p < 0.03). These findings show that T lymphocytes of patients with PBD recognize a wide range of bird proteins, which induce marked T-cell proliferation. Copyright © 1996 by Mosby-Year Book, Inc.
Filiaciones:
Mendoza F.:
Institute Nacional de Enfermedades Respiratorias, Facultad de Medicina UNAM, Unidad Xochimilco UAM México
Melendro E.I.:
Institute Nacional de Enfermedades Respiratorias, Facultad de Medicina UNAM, Unidad Xochimilco UAM México
Baltazares M.:
Institute Nacional de Enfermedades Respiratorias, Facultad de Medicina UNAM, Unidad Xochimilco UAM México
Banales J.L.:
Institute Nacional de Enfermedades Respiratorias, Facultad de Medicina UNAM, Unidad Xochimilco UAM México
Ximénez C.:
Institute Nacional de Enfermedades Respiratorias, Facultad de Medicina UNAM, Unidad Xochimilco UAM México
Chapela R.:
Institute Nacional de Enfermedades Respiratorias, Facultad de Medicina UNAM, Unidad Xochimilco UAM México
Selman M.:
Institute Nacional de Enfermedades Respiratorias, Facultad de Medicina UNAM, Unidad Xochimilco UAM México
Instituto Nacional de Enfermedades Respiratorias, Col. Sección XVI, Tlalpan 4502, CP. 14080, México DF, Mexico
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