Reproducibility of pilot scale Xanthan fermentations
Por:
Amanullah A., Serrano L.-C., Galindo E., Nienow A.W.
Publicada:
1 ene 1996
Resumen:
The reproducibility of results is an essential prerequisite for a successful systematic analysis of the influence of process development parameters on fermentation performance. Concerns of inoculum inconsistency and strain instability in Xanthan fermentations have led to the use of a highly productive variant of Xanthomonas campestris NRRL-B1459 under strict preservation protocols and the development of an improved, yet simple inoculation strategy. This technique allows the propagation of standardized inocula, ensuring that starter cultures both identical in concentration and history are used. Implementation of this methodology resulted in extremely reproducible cultivations in shake flask and in fermenters of up to a pilot scale of 150 L in volume.
Filiaciones:
Amanullah A.:
BBSRC Ctr. for Biochem. Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
Serrano L.-C.:
Departamento de Bioingeniería, Instituto de Biotecnología, Univ. Nac. Autónome de Mex., Apdo. Post 510-3, Cuernavaca, Morelos 62271, Mexico
Galindo E.:
Departamento de Bioingeniería, Instituto de Biotecnología, Univ. Nac. Autónome de Mex., Apdo. Post 510-3, Cuernavaca, Morelos 62271, Mexico
Nienow A.W.:
BBSRC Ctr. for Biochem. Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
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