Salmonella typhimurium infection in mice: Lymphoproliferative responses to fractionated protein antigens
Por:
Melendro E.I., Calderon G.M.A., Perez C., Ximenez C.
Publicada:
1 ene 1995
Resumen:
The proliferative response of spleen cells of mice immunized with S. typhimurium by the oral route was analyzed using antigen fractions from a protein extract of the bacteria. Mice that survived the challenge with a virulent strain of Salmonella and normal mice were also studied. Mice were immunized with three doses of live S. typhimurium on consecutive days (3C) or once a week for 3 weeks (3W). Fractions 12-100 kDa from the protein extract were separated by SDS-polyacrylamide gel electrophoresis, electroblotted to nitrocellulose membranes and processed to obtain particulate antigens for use in lymphocyte cultures. Mice immunized weekly showed a higher survival rate and responded to more antigenic fractions. We identified three fractions of 68-76, 50-52, and 42-45 kDa that were immunodominant for spleen cells from S. typhimurium immunized mice and from survivors to the challenge with the virulent strain.
Filiaciones:
Melendro E.I.:
Depto de Medicina Experimental, Edificio 'A', 6to piso, Facultad de Medicina, UNAM, Aptdo. Postal 70641, Mexico D.F., Mexico
Calderon G.M.A.:
Depto de Medicina Experimental, Edificio 'A', 6to piso, Facultad de Medicina, UNAM, Aptdo. Postal 70641, Mexico D.F., Mexico
Perez C.:
Depto de Medicina Experimental, Edificio 'A', 6to piso, Facultad de Medicina, UNAM, Aptdo. Postal 70641, Mexico D.F., Mexico
Ximenez C.:
Depto de Medicina Experimental, Edificio 'A', 6to piso, Facultad de Medicina, UNAM, Aptdo. Postal 70641, Mexico D.F., Mexico
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