Redox imbalance at the start of each morphogenetic step of Neurospora crassa conidiation


Por: Toledo I., Rangel P., Hansberg W.

Publicada: 1 ene 1995
Resumen:
The conidiation process of Neurospora crassa is characterized by three morphogenetic steps: hyphal adhesion, aerial hyphal formation, and production of conidia. Total protein oxidation and specific enzyme oxidation coincided with an increased oxygen-dependent chemiluminescence and indicated the occurrence of a hyperoxidant state at the onset of all three morphogenetic steps. Oxidation of NAD(P)H and excretion of glutathione disulfide was detected at the start of hyphae adhesion. Here we show that NAD(P)H and glutathione redox imbalance also occurred at the beginning of aerial hyphal growth and just before formation of conidia in the isolated cell structures. An increased loss and oxidation of NAD(P)(H) and glutathione were detected with each morphogenetic transition. These results give further support to our proposal that a hyperoxidant state develops at the start of each of the three morphogenetic processes during N. crassa conidiation.

Filiaciones:
Toledo I.:
 Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Ap. Postal 565-A, Cuernavaca 62280, Mexico

Rangel P.:
 Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ap. Postal 70-242, 04510 México, DF, Mexico

Hansberg W.:
 Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ap. Postal 70-242, 04510 México, DF, Mexico
ISSN: 00039861
Editorial
ELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA, Estados Unidos America
Tipo de documento: Article
Volumen: 319 Número: 2
Páginas: 519-524
WOS Id: A1995RC22100025
ID de PubMed: 7786037