Hormone-dependent transactivation by the human androgen receptor is regulated by a dnaJ protein
Por:
Caplan A.J., Langley E., Wilson E.M., Vidal J.
Publicada:
1 ene 1995
Resumen:
Genetic studies were performed to examine the role of eukaryotic dnaJ protein, Ydj1p, in the regulated activation of human androgen receptor (hAR) after heterologous expression in Saccharomyces cerevisiae. Hormone-dependent activation of bAR was measured as a function of lacZ reporter gene expression, which was defective in ydj1-151 and ydj1-2(?) null mutant strains compared to the wild type. This defect was not due to receptor misfolding, since hAR in both wild type and mutant strains had a similar capacity to bind hormone. The target for Ydj1p action was determined to be the hAR hormone binding domain since an N-terminal fragment lacking this region was constitutively active in both wild type and ydj1-151 mutant strains. These data correlate hormone dependence of bAR activation with a requirement for Ydj1p function and are consistent with a role for dnaJ proteins in signal transduction by steroid hormone receptors.
Filiaciones:
Caplan A.J.:
Department of Cell Biology/Anatomy, Mount Sinai Medical Center, New York, NY 10029, United States
Langley E.:
Department of Cell Biology/Anatomy, Mount Sinai Medical Center, New York, NY 10029, United States
Wilson E.M.:
Department of Cell Biology/Anatomy, Mount Sinai Medical Center, New York, NY 10029, United States
Vidal J.:
Department of Cell Biology/Anatomy, Mount Sinai Medical Center, New York, NY 10029, United States
Hybrid Gold
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