A significant fraction of calcium transients in intact guinea pig ventricular myocytes is mediated by Na+Ca2+ exchange
Por:
Santi C.M., Connor J.A., Hernández-Cruz A.
Publicada:
1 ene 1995
Resumen:
Ca2+ mobilization elicited by simulation with brief pulses of high K + were monitored with confocal laser scanned microscopy in intact, guinea pig cardiac myocytes loaded with the calcium indicator fluo-3. Single wavelength ratioing of fluorescence images obtained after prolonged integration times revealed non-uniformities of intracellular Ca2+ changes across the cell, suggesting the presence of significant spatial Ca2+ gradients. Treatment with 20 ?M ryanodine, an inhibitor of Ca2+ release from the SR, and 10 ?M verapamil, a calcium channel blocker, reduced by 42% and 76% respectively the changes in [Ca2+]i elicited by membrane depolarization. The overall spatial distribution of [Ca2+]i changes appeared unchanged. Ca2+ transients recorded in the presence of verapamil and ryanodine (about 20% of the size of control responses); diminished in the presence of 50 ?M 2-4 Dichlorbenzamil (DCB) or 5 mM nickel, two relatively specific inhibitors of the Na+/Ca2+ exchange mechanism. Conversely, when the reversal potential of the Na+/Ca2+ exchange was shifted to negative potentials by lowering [NA+]0 or by increasing [Na+]i by treatment with 20 ?M monensin, the amplitude of these Ca2+ transients increased. Ca2+ transients elicited by membrane depolarization and largely mediated by reverse operation of Na+Ca2+ exchange could be recorded in the presence of ryanodine, verapamil and monensin. These findings suggest that in intact guinea pig cardiac cells, Ca2+ influx through the Na+/Ca2+ exchange mechanism activated by a membrane depolarization in the physiological range can be sufficient to play a significant role in excitation-contraction coupling. © 1995.
Filiaciones:
Santi C.M.:
Departamento de Neurociencias, Instituto de Fisiología Celular, UNAM, 04510 México City, Mexico
Connor J.A.:
Department of Neurosciences, Roche Institute of Molecular Biology, Nutley, NJ 07110, United States
Hernández-Cruz A.:
Department of Neurosciences, Roche Institute of Molecular Biology, Nutley, NJ 07110, United States
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