Continuous production of 5'-ribonucleotides from yeast RNA by hydrolysis with immobilized 5'-phosphodiesterase and 5'-adenylate deaminase
Por:
Olmedo F., Iturbe F., Gomez-Hernández J., López-Munguía A.
Publicada:
1 ene 1994
Resumen:
The production of 5?-IMP and 5?-GMP by enzymatic conversion from RNA using a continuous two packed-bed reactor was investigated. 5?-Phosphodiesterase (5?PD) and 5?-adenylate deaminase (5?AD) were immobilized in an acrylic resin to produce derivatives with about 15 U/g of support. The kinetic properties of the enzymes were described by Michaelis-Menten models: no significant differences were found in the Km value of the free and immobilized 5?AD (60 and 20 ?m, respectively), whereas for 5?PD the Km value was one order of magnitude higher for the immobilized enzyme (4.85 mg RNA/ml), probably due to diffusional limitations. Both enzymes remained stable after 8 h of use in a continuous packed-bed reactor whereas the half lives of the free enzymes were 193 min and 240 min at 40°C and 70°C for 5?AD and 5?PD, respectively. A procedure is proposed for the design of a continuous two packed-bed column process. © 1994 Rapid Science Publishers.
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