Qualitative and quantitative examination of rat and human fetal dopaminergic grafts
Por:
Staal M.J., Hogen Esch R.I., Tomasini R., Kraayenbrink R., Kema I.P., Buijs R.M., Go K.G., Zuiderveen F., Buys C.H.C.M.
Publicada:
1 ene 1990
Resumen:
This study was carried out as a prelude to possible implantations of cultured human fetal dopaminergic grafts in parkinsonian patients. Examination of fetal rat ventral mesencephalon tissue for morphology, viability, and dopamine content showed an optimal gestational age for neural grafting in rat experiments of approximately 17 days. Moreover, fetal rat ventral mesencephalon tissue was cultured, and neural dopaminergic cells were observed in cell culture in 4 (11 %) out of 36 ventral mesencephalon specimens derived from 15- to 21-day-old rat fetuses. Human fetal donor material from elective abortions was examined for morphology and cell culture possibilities. In 48 curettements, fetal tissue was seen in 34 (71%) of these, resulting in 17 (50%) cultures containing dopaminergic cells. Both fetal rat and human cell cultures were continued for approximately 8 weeks and appeared to remain positive upon immunocyto chemical dopamine staining. © 1990 S. Karger AG, Basel.
Filiaciones:
Staal M.J.:
University/Academic Hospital, Groningen, Netherlands
Hogen Esch R.I.:
University/Academic Hospital, Groningen, Netherlands
Tomasini R.:
University/Academic Hospital, Groningen, Netherlands
Kraayenbrink R.:
University/Academic Hospital, Groningen, Netherlands
Kema I.P.:
University/Academic Hospital, Groningen, Netherlands
Buijs R.M.:
Netherlands’ Institute for Brain Research, Amsterdam, Netherlands
Go K.G.:
University/Academic Hospital, Groningen, Netherlands
Zuiderveen F.:
University/Academic Hospital, Groningen, Netherlands
Buys C.H.C.M.:
University/Academic Hospital, Groningen, Netherlands
Netherlands’ Institute for Brain Research, Amsterdam, Netherlands
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