Inhibition of mitogenesis induced by phytohemagglutininand Lens culinaris lectin in adherent-cell supernatants treated with protein extract of Mycobacterium tuberculosis
Por:
Parra C., Montano L.F., Huesca M., Rayón I., Willms K., Goodsaid F.
Publicada:
1 ene 1986
Resumen:
Specific stimulation of T cells by phytohemagglutinin and Lens culinaris lectin was inhibited by a soluble factor(s) secreted by normal adherent cells stimulated with culture filtrate protein extract (CFPE) derived from bacterial cultures of Mycobacterium tuberculosis H37Ra (avirulent) and H37Rv (virulent). The induction of the inhibitory factor was blocked by the presence of hyperimmune antisera to H37Rv or H37Ra CFPE. The inhibitory factor did not seem to be a CFPE reprocessed by the adherent cells. Inhibitory activity was maximal in supernatants of adherent-cell cultures incubated for 48 h; the inhibitory factor was heat labile, and its production was dependent on the concentration of M. tuberculosis CFPE. A mouse monocyte-macrophage cell line, ATCC J774A.1, produced an identical inhibitory factor, thus excluding a non-macrophage-contaminating adherent cell as the source of the factor. This inhibitory factor also interfered with the recognition of phytohemagglutinin and Lens culinaris lectin by T cells.
Filiaciones:
Parra C.:
Departmento de Inmunología, Instituto Nacional de Enfermedades Respiratorias, Calz. Tlalpan 4502, C. P. 14080, Mexico
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autonoma de México, C. U. 04510, D. F.2, Mexico
Huesca M.:
Departmento de Inmunología, Instituto Nacional de Enfermedades Respiratorias, Calz. Tlalpan 4502, C. P. 14080, Mexico
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autonoma de México, C. U. 04510, D. F.2, Mexico
Rayón I.:
Departmento de Inmunología, Instituto Nacional de Enfermedades Respiratorias, Calz. Tlalpan 4502, C. P. 14080, Mexico
Willms K.:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autonoma de México, C. U. 04510, D. F.2, Mexico
Goodsaid F.:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autonoma de México, C. U. 04510, D. F.2, Mexico
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