Kainate reversibly aggregates brain tubulin in vitro
Por:
López-Colomé A.M., Casas A.
Publicada:
1 ene 1984
Resumen:
The effect of kainate, an heterocyclic analogue of glutamate on tubulin polimerization was studied. We demonstrate that kainate induces a dose-dependent aggregation of rat brain tubulin either purified by DEAE-Sephadex A-50 or in the presence of MAPs into a mesh-like structure. Such polymer is cold-, CaCl2- and colchicine-insensitive. Removal of kainate from the incubation medium yields free tubulin competent for polimerizing into normal microtubules in the presence of GTP. © 1984.
Filiaciones:
López-Colomé A.M.:
Departmento de Neurociencias, Centro de Investigaciones en Fisiología Celular, UNAM, 04510 México, D.F., Mexico
Casas A.:
Departmento de Neurociencias, Centro de Investigaciones en Fisiología Celular, UNAM, 04510 México, D.F., Mexico
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