Neurospora crassa glutamine synthetase. Purification by affinity chromatography and characterization of subunit structure.
Por:
Palacios R.
Publicada:
1 ene 1976
Resumen:
Neurospora crassa glutamine synthetase was purified to homogeneity by a procedure based on affinity chromatography. The enzyme is adsorbed to a matrix of anthranilic acid bound to Sepharose and eluted with AMP. Different experimental approaches indicate that the enzyme has an octameric structure formed by subunits of identical molecular weight.