Inositol biosynthesis in Neurospora crassa
Por:
Piña E., Tatum E.L.
Publicada:
1 ene 1967
Resumen:
1. 1. The biosynthesis of myo-inositol in wild-type Neurospora crassa and in inositol-less mutant 896001 has been studied. 2. 2. Two enzyme preparations capable of synthesizing myo-inositol were obtained, separated and partially purified. One of them, which has been purified 360-fold, is present only in the wild-type strain and converts glucose 6-phosphate into myo-inositol phosphate, with an absolute requirement for oxidized nicotinamide-adenine dinucleotide. A phosphatase which hydrolyzes myo-inositol phosphate has been separated from this enzymatic system. 3. 3. The other enzyme, purified only 8-fold, is present both in the wild-type strain and the inositol-less mutant. This enzyme, an inositol dehydrogenase, forms myo-inositol from DL-epi-inosese-2 and reduced nicotinamide-adenine dinucleotide phosphate. 4. 4. It is concluded that in Neurospora crassa: (a) myo-inositol is normally synthesized from glucose 6-phosphate; (b) epi-inosese is not synthesized under normal conditions; and (c) epi-inosose is not an intermediate in the enzymatic conversion of glucose 6-phosphate to myo-inositol phosphate. © 1967.
Filiaciones:
Piña E.:
Rockefeller University, New York, NY, United States
Tatum E.L.:
Rockefeller University, New York, NY, United States
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