Assessment of the effects of oseltamivir and indomethacin on dopamine, 5-HIAA, and some oxidative stress markers in stomach and brain of Salmonella typhimurium-infected rats
Por:
Calderon Guzman, David, Ortiz Herrera, Maribel, Osnaya Brizuela, Norma, Barragan Mejia, Gerardo, Trujillo Jimenez, Francisca, Hernandez Garcia, Ernestina, Jurarez Olguin, Hugo
Publicada:
1 ene 2016
Resumen:
OBJECTIVES: The purpose of this study was to measure the effect of
oseltamivir and indomethacin on dopamine and 5-HIAA levels and some
oxidative biomarkers in brain and stomach of young rats in conditions of
infection.
METHODS: Female Sprague Dawley rats in absence or presence of a live
culture of Salmonella typhimurium (S. Typh), were treated as follows:
PBS, group 1 (control); oseltamivir (100 mg/kg), group 2; indomethacin
(67 mu g/kg) group 3; oseltamivir (100 mg/kg) + indomethacin (67 mu
g/kg), group 4. The drugs were administered intraperitoneally every 24
hr for 5 days while S. Typh was give orally in the first and third day.
C-reactive proteins was measured in blood on sacrifice, and from brain
extract, dopamine and 5-HIAA levels as well as GSH, calcium, and H2O2
and total ATPase activity were measured by validated methods.
RESULTS: Dopamine increased significantly in cortex and
cerebellum/medulla oblongata of groups that received indomethacin and
oseltamivir. 5-HIAA increased significantly in all groups that received
S. Typh. H2O2 decreased significantly in cortex regions of animals that
received oseltamivir and indomethacin in presence of S. Typh. Total
ATPase increased significantly in cortex and hemispheres of groups that
received oseltamivir as well as in cerebellum/medulla oblongata and
stomach of animals that received oseltamivir and indomethacin combined
with S. Typh. GSH increased and calcium decreased significantly in
stomach of animals that received oseltamivir or indomethacin alone or
combined with S. Typh.
CONCLUSION: These results demonstrate the association between
inflammatory response, oxidative stress, dopaminergic, and serotonergic
metabolism in an experimental inflammatory animal model.
Filiaciones:
Calderon Guzman, David:
Inst Nacl Pediat, Lab Neuroquim, Ave Iman 1,3rd piso, Mexico City 04530, DF, Mexico
Ortiz Herrera, Maribel:
Lab Bacteriol Expt INP, Mexico City, DF, Mexico
Osnaya Brizuela, Norma:
Inst Nacl Pediat, Lab Neuroquim, Ave Iman 1,3rd piso, Mexico City 04530, DF, Mexico
Barragan Mejia, Gerardo:
Inst Nacl Pediat, Lab Neuroquim, Ave Iman 1,3rd piso, Mexico City 04530, DF, Mexico
Trujillo Jimenez, Francisca:
Univ Nacl Autonoma Mexico, Lab Farmacol INP, Fac Med, Mexico City 04510, DF, Mexico
Hernandez Garcia, Ernestina:
Univ Nacl Autonoma Mexico, Lab Farmacol INP, Fac Med, Mexico City 04510, DF, Mexico
Jurarez Olguin, Hugo:
Univ Nacl Autonoma Mexico, Lab Farmacol INP, Fac Med, Mexico City 04510, DF, Mexico
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