Assessment of the effects of oseltamivir and indomethacin on dopamine, 5-HIAA, and some oxidative stress markers in stomach and brain of Salmonella typhimurium-infected rats


Por: Calderon Guzman, David, Ortiz Herrera, Maribel, Osnaya Brizuela, Norma, Barragan Mejia, Gerardo, Trujillo Jimenez, Francisca, Hernandez Garcia, Ernestina, Jurarez Olguin, Hugo

Publicada: 1 ene 2016
Resumen:
OBJECTIVES: The purpose of this study was to measure the effect of oseltamivir and indomethacin on dopamine and 5-HIAA levels and some oxidative biomarkers in brain and stomach of young rats in conditions of infection. METHODS: Female Sprague Dawley rats in absence or presence of a live culture of Salmonella typhimurium (S. Typh), were treated as follows: PBS, group 1 (control); oseltamivir (100 mg/kg), group 2; indomethacin (67 mu g/kg) group 3; oseltamivir (100 mg/kg) + indomethacin (67 mu g/kg), group 4. The drugs were administered intraperitoneally every 24 hr for 5 days while S. Typh was give orally in the first and third day. C-reactive proteins was measured in blood on sacrifice, and from brain extract, dopamine and 5-HIAA levels as well as GSH, calcium, and H2O2 and total ATPase activity were measured by validated methods. RESULTS: Dopamine increased significantly in cortex and cerebellum/medulla oblongata of groups that received indomethacin and oseltamivir. 5-HIAA increased significantly in all groups that received S. Typh. H2O2 decreased significantly in cortex regions of animals that received oseltamivir and indomethacin in presence of S. Typh. Total ATPase increased significantly in cortex and hemispheres of groups that received oseltamivir as well as in cerebellum/medulla oblongata and stomach of animals that received oseltamivir and indomethacin combined with S. Typh. GSH increased and calcium decreased significantly in stomach of animals that received oseltamivir or indomethacin alone or combined with S. Typh. CONCLUSION: These results demonstrate the association between inflammatory response, oxidative stress, dopaminergic, and serotonergic metabolism in an experimental inflammatory animal model.

Filiaciones:
Calderon Guzman, David:
 Inst Nacl Pediat, Lab Neuroquim, Ave Iman 1,3rd piso, Mexico City 04530, DF, Mexico

Ortiz Herrera, Maribel:
 Lab Bacteriol Expt INP, Mexico City, DF, Mexico

Osnaya Brizuela, Norma:
 Inst Nacl Pediat, Lab Neuroquim, Ave Iman 1,3rd piso, Mexico City 04530, DF, Mexico

Barragan Mejia, Gerardo:
 Inst Nacl Pediat, Lab Neuroquim, Ave Iman 1,3rd piso, Mexico City 04530, DF, Mexico

Trujillo Jimenez, Francisca:
 Univ Nacl Autonoma Mexico, Lab Farmacol INP, Fac Med, Mexico City 04510, DF, Mexico

Hernandez Garcia, Ernestina:
 Univ Nacl Autonoma Mexico, Lab Farmacol INP, Fac Med, Mexico City 04510, DF, Mexico

Jurarez Olguin, Hugo:
 Univ Nacl Autonoma Mexico, Lab Farmacol INP, Fac Med, Mexico City 04510, DF, Mexico
ISSN: 0172780X
Editorial
MAGHIRA & MAAS PUBLICATIONS, PO BOX 26132, S-100 41 STOCKHOLM, SWEDEN, Suecia
Tipo de documento: Article
Volumen: 37 Número: 2
Páginas: 129-136
WOS Id: 000391807100008
ID de PubMed: 27179576