Electrophysiological activity of a neurotoxic fraction from the venom of box jellyfish Carybdea marsupialis
Por:
Lazcano-Pérez F., Arellano R.O., Garay E., Arreguín-Espinosa R., Sánchez-Rodríguez J.
Publicada:
1 ene 2017
Resumen:
Carybdea marsupialis is a widely distributed box jellyfish found in the
Mediterranean and in the tropical waters of the Caribbean Sea. Its venom
is a complex mixture of biologically active compounds that are used to
catch prey. In order to evaluate the activity of the neurotoxins in the
venom, bioassays were carried out using the marine crab Ocypode
quadrata. The proteins with neurotoxic effect were partially purified
using low-pressure liquid chromatography techniques. Gel filtration
(Sephadex G-50M) was used as the first step and the active fraction in
crabs was passed through a QAE Sephadex A-25 column. Finally, the active
fraction was run onto a Fractogel EMD SO3- column. No further
purification step could be carried out due to the loss of neurotoxic
activity. The Fractogel EMD SW fraction was analyzed
electrophysiologically using the voltage-clamp technique in Xenopus
laevis oocytes expressing membrane proteins from rat brain through mRNA
injection. The crude venom and a fraction were observed to affect
crustaceans and showed at least two types of bioactivity in oocytes
expressing brain proteins. The effects were dose-dependent and
completely reversible. These results evidence the presence of
neurotoxins in Carybdea marsupialis venom that act on membrane proteins
of the vertebrate nervous system. (C) 2016 Elsevier Inc. All rights
reserved.
Filiaciones:
Lazcano-Pérez F.:
Univ Nacl Autonoma Mexico, Unidad Acad Sistemas Arrecifales Puerto Morelos, Inst Ciencias Mar & Limnol, Prolongac Ninos Heroes S-N, Puerto Morelos 77580, Quintana Roo, Mexico
Univ Nacl Autonoma Mexico, Inst Quim, Ciudad De Mexico 04510, DF, Mexico
Univ Autonoma Metropolitana, Dept Ciencias Salud, Campus Iztapalapa, Ciudad De Mexico 09340, DF, Mexico
Unidad Académica de Sistemas Arrecifales Puerto Morelos, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Prolongación Niños Héroes s/n, Domicilio conocido, Puerto Morelos, Quintana Roo 77580, Mexico
Instituto de Química, Instituto de Química, Universidad Nacional Autónoma de México, Ciudad de México, 04510, Mexico
Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana, Campus Iztapalapa, Ciudad de México, 09340, Mexico
Arellano R.O.:
Univ Nacl Autonoma Mexico, Inst Neurobiol, Juriquilla 76001, Queretaro, Mexico
Instituto de Neurobiología, Universidad Nacional Autónoma de México, Juriquilla, Querétaro 76001, Mexico
Garay E.:
Univ Nacl Autonoma Mexico, Inst Neurobiol, Juriquilla 76001, Queretaro, Mexico
Instituto de Neurobiología, Universidad Nacional Autónoma de México, Juriquilla, Querétaro 76001, Mexico
Arreguín-Espinosa R.:
Univ Nacl Autonoma Mexico, Inst Quim, Ciudad De Mexico 04510, DF, Mexico
Instituto de Química, Instituto de Química, Universidad Nacional Autónoma de México, Ciudad de México, 04510, Mexico
Sánchez-Rodríguez J.:
Univ Nacl Autonoma Mexico, Unidad Acad Sistemas Arrecifales Puerto Morelos, Inst Ciencias Mar & Limnol, Prolongac Ninos Heroes S-N, Puerto Morelos 77580, Quintana Roo, Mexico
Unidad Académica de Sistemas Arrecifales Puerto Morelos, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Prolongación Niños Héroes s/n, Domicilio conocido, Puerto Morelos, Quintana Roo 77580, Mexico
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