Identification of Pseudomonas syringae pv. phaseolicola as the causal agent of halo blight in yellow beans in northern Sinaloa, Mexico
Por:
Felix-Gastelum, R., Maldonado-Mendoza, I. E., Navarrete-Maya, R., Olivas-Peraza, N. G., Brito-Vega, H., Acosta-Gallegos, J. A.
Publicada:
1 jul 2016
Resumen:
Yellow beans are produced under irrigation during the fall-winter
growing season in the coastal lowlands of Sinaloa, Mexico. Halo blight,
is the most important disease during the winter time. The objectives of
this study were to: a) identify the causal agent of the disease and b)
determine the physiological races of the bacterium. Twelve isolates of
the bacterium associated with infected leaves and pods from the Azufrado
Higuera cultivar were obtained from December 2013 through January 2014.
Biochemical, physiological and molecular characteristics of the
bacterial isolates coincide with those of Pseudomonas syringae pv.
phaseolicola (Psph) reported in the highlands of Mexico. All twelve
isolates of the bacterium from bean were inoculated under greenhouse
conditions and caused similar symptoms as those observed in the field.
The genes rpoB and rpoD were sequenced for phylogenetic analysis and
compared to databases to confirm their identities. These markers were
thus used to determine that the 12 analyzed isolates are identical, and
are similar to the Psph sequences currently available in databases.
Compatibility patterns between bean cultivars from the differential set
revealed that six out of the twelve isolates infected all differential
plants and matched the pattern of race six. In contrast, the remaining
isolates displayed inconclusive compatibility patterns, indicating that
the differential set does not provide conclusive information for
pathotype identification. This suggests that the current differential
set of genotypes requires the inclusion of novel bean genotypes, in
order to explore the existence of potential new races of this pathogen.
Filiaciones:
Felix-Gastelum, R.:
Univ Occidente, Dept Ciencias Biol, Unidad Los Mochis, Blvd Macario Gaxiola & Carretera Int S-N, Los Mochis 81223, Sinaloa, Mexico
Maldonado-Mendoza, I. E.:
Inst Politecn Nacl, Dept Biotecnol Agr, CIIDIR Unidad Sinaloa, Blvd Juan de Dios Batiz Paredes 250, Guasave 81101, Sinaloa, Mexico
Navarrete-Maya, R.:
Univ Nacl Autonoma Mexico, UNIGRAS, Fac Estudios Super Cuautitlan, Cuautitlan 54700, Estado De Mexic, Mexico
Olivas-Peraza, N. G.:
Univ Occidente, Dept Ciencias Biol, Unidad Los Mochis, Blvd Macario Gaxiola & Carretera Int S-N, Los Mochis 81223, Sinaloa, Mexico
Brito-Vega, H.:
Inst Politecn Nacl, Dept Biotecnol Agr, CIIDIR Unidad Sinaloa, Blvd Juan de Dios Batiz Paredes 250, Guasave 81101, Sinaloa, Mexico
Acosta-Gallegos, J. A.:
SAGARPA, INIFAP, Campo Expt Bajio, AP 112, Guanajuato 38110, Mexico
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